Overall, the sequencing generated 43,834 reads, with an N50 value of 12,958 bp. Nanopore library adaptors were trimmed with Porechop software (v0.2.4) (4). The filtration by NanoFilt software (v2.8.0) (5) and removing of adaptors generated 35,699 reads. The long reads were assembled using the de novo assembler Flye (v2.9.3-b1797) (6), yielding two contigs. Polishing of the two contigs was performed using four rounds of racon (v1.5.0) (7) and a single round of medaka (v2.0.1) (8). The assembly statistics were generated using QUAST (v5.2.0) (9), while the completeness of the genomes was
assessed using BUSCO (v5.5.0) (10). However, assembly using this strategy may contain a high number of errors and frameshifts. Default parameters were used for all software. The first contig constitutes a circular chromosome of 6,193,318 bp with 62.5% GC content. The second contig is a circular plasmid containing 394,003 bp with a GC content of 60.5%. The circularity was verified through assembly. The annotation was carried out using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) (v6.8) (11).