Draft de novo assemblies were generated with Flye v. 2.9.2, setting the parametersmetaand–extra-params min_read_cov_cutoff=10. The draft assemblies were subsequently polished twice with Medaka v. 1.11.3 (Oxford Nanopore Technologies, Oxford, United Kingdom). Assembly graphs were inspected with Bandage v. 0.8.1. Platon v. 1.7 was used to classify plasmids. SemiBin2 v. 2.0.2 was implemented as an independent binning approach using a self-supervised learning model (–sequencing-type long_read–minfasta-kbs 1500). Completeness and contamination levels were assessed using CheckM2 v. 1.0.1. Genome chimerism was evaluated using GUNC v. 1.0.6.