Atabeyarchaeia classis nov.

The type species for this taxonomic group were initially reconstructed by manual curation of Illumina short-read assemblies and then validated using both Nanopore and PacBio long reads. Phylogenetic analyses of this fully curated genome using several sets of marker genes placed the complete genomes in a monophyletic group within the Asgard clade as a sister group to Atabeyarchaeia. We performed phylogenetic analyses using concatenated marker sets of 47 archaeal clusters of orthologous genes (arCOGs) and 15 ribosomal protein (RP15) gene cluster, as well as the 16S rRNA gene. The new genomes share only <45% AAI when compared to other Asgard genomes, consistent with their assignment to a new phylum. Although our analyses provide evidence for distinction at the phylum level, we chose to adhere to the Genome Taxonomy Database (GTDB) for standardized microbial genome nomenclature. We establish a robust classification and propose Freyarchaeum as the type genus for the family Freyarchaeaceae, the order Freyarchaeales, the class Freyarchaeia with the Asgardarchaeota phylum. Based on protein content and compositional features, we infer that Freyarchaeia is a non-methanogenic acetogenic chemoheterotroph, with potential to grow lithoautotrophically by using H2 as an electron donor. This lineage was originally reconstructed and named from Guaymas Basin, located in the Gulf of California, México, and from Jinze Hot Spring (Yunnan, China). Subsequently, another group used the original data to recover similar genomes and referred to them as Jordarchaeia. Here, we retain the original nomenclature.

Based on protein content and compositional features, we infer that Atabeyarchaeia is a non-methanogenic acetogenic chemoheterotroph, with potential to grow lithoautotrophically by using H2 as an electron donor. Phylogenetic analyses using several sets of marker genes placed two complete genomes in a monophyletic group within the Asgard clade as a sister group to Freyarchaeia. We performed phylogenetic analyses using concatenated marker sets of 47 archaeal clusters of orthologous genes (arCOGs) and 15 ribosomal protein (RP15) gene cluster, as well as the 16S rRNA gene. The new genomes share only 40–45% AAI when compared to other Asgard genomes, consistent with their assignment to a new phylum. Although our analyses provide evidence for distinction at the phylum level, we chose to adhere to the Genome Taxonomy Database (GTDB) for standardized microbial genome nomenclature. We establish a robust classification and propose Atabeyarchaeum as the type genus for the family Atabeyarchaeaceae, the order Atabeyarchaeales, the class Atabeyarchaeia with the Asgardarchaeota phylum.

The type species for this taxonomic group were initially reconstructed by manual curation of Illumina short-read assemblies and then validated using both Nanopore and PacBio long reads. Phylogenetic analyses of this fully curated genome using several sets of marker genes placed the complete genomes in a monophyletic group within the Asgard clade as a sister group to Atabeyarchaeia. We performed phylogenetic analyses using concatenated marker sets of 47 archaeal clusters of orthologous genes (arCOGs) and 15 ribosomal protein (RP15) gene cluster, as well as the 16S rRNA gene. The new genomes share only <45% AAI when compared to other Asgard genomes, consistent with their assignment to a new phylum. Although our analyses provide evidence for distinction at the phylum level, we chose to adhere to the Genome Taxonomy Database (GTDB) for standardized microbial genome nomenclature. We establish a robust classification and propose Freyarchaeum as the type genus for the family Freyarchaeaceae, the order Freyarchaeales, the class Freyarchaeia with the Asgardarchaeota phylum. Based on protein content and compositional features, we infer that Freyarchaeia is a non-methanogenic acetogenic chemoheterotroph, with potential to grow lithoautotrophically by using H2 as an electron donor. This lineage was originally reconstructed and named from Guaymas Basin, located in the Gulf of California, México, and from Jinze Hot Spring (Yunnan, China). Subsequently, another group used the original data to recover similar genomes and referred to them as Jordarchaeia. Here, we retain the original nomenclature.

Based on protein content and compositional features, we infer that Atabeyarchaeia is a non-methanogenic acetogenic chemoheterotroph, with potential to grow lithoautotrophically by using H2 as an electron donor. Phylogenetic analyses using several sets of marker genes placed two complete genomes in a monophyletic group within the Asgard clade as a sister group to Freyarchaeia. We performed phylogenetic analyses using concatenated marker sets of 47 archaeal clusters of orthologous genes (arCOGs) and 15 ribosomal protein (RP15) gene cluster, as well as the 16S rRNA gene. The new genomes share only 40–45% AAI when compared to other Asgard genomes, consistent with their assignment to a new phylum. Although our analyses provide evidence for distinction at the phylum level, we chose to adhere to the Genome Taxonomy Database (GTDB) for standardized microbial genome nomenclature. We establish a robust classification and propose Atabeyarchaeum as the type genus for the family Atabeyarchaeaceae, the order Atabeyarchaeales, the class Atabeyarchaeia with the Asgardarchaeota phylum.

The type species for this taxonomic group were initially reconstructed by manual curation of Illumina short-read assemblies and then validated using both Nanopore and PacBio long reads. Phylogenetic analyses of this fully curated genome using several sets of marker genes placed the complete genomes in a monophyletic group within the Asgard clade as a sister group to Atabeyarchaeia. We performed phylogenetic analyses using concatenated marker sets of 47 archaeal clusters of orthologous genes (arCOGs) and 15 ribosomal protein (RP15) gene cluster, as well as the 16S rRNA gene. The new genomes share only <45% AAI when compared to other Asgard genomes, consistent with their assignment to a new phylum. Although our analyses provide evidence for distinction at the phylum level, we chose to adhere to the Genome Taxonomy Database (GTDB) for standardized microbial genome nomenclature. We establish a robust classification and propose Freyarchaeum as the type genus for the family Freyarchaeaceae, the order Freyarchaeales, the class Freyarchaeia with the Asgardarchaeota phylum. Based on protein content and compositional features, we infer that Freyarchaeia is a non-methanogenic acetogenic chemoheterotroph, with potential to grow lithoautotrophically by using H2 as an electron donor. This lineage was originally reconstructed and named from Guaymas Basin, located in the Gulf of California, México, and from Jinze Hot Spring (Yunnan, China). Subsequently, another group used the original data to recover similar genomes and referred to them as Jordarchaeia. Here, we retain the original nomenclature.

Based on protein content and compositional features, we infer that Atabeyarchaeia is a non-methanogenic acetogenic chemoheterotroph, with potential to grow lithoautotrophically by using H2 as an electron donor. Phylogenetic analyses using several sets of marker genes placed two complete genomes in a monophyletic group within the Asgard clade as a sister group to Freyarchaeia. We performed phylogenetic analyses using concatenated marker sets of 47 archaeal clusters of orthologous genes (arCOGs) and 15 ribosomal protein (RP15) gene cluster, as well as the 16S rRNA gene. The new genomes share only 40–45% AAI when compared to other Asgard genomes, consistent with their assignment to a new phylum. Although our analyses provide evidence for distinction at the phylum level, we chose to adhere to the Genome Taxonomy Database (GTDB) for standardized microbial genome nomenclature. We establish a robust classification and propose Atabeyarchaeum as the type genus for the family Atabeyarchaeaceae, the order Atabeyarchaeales, the class Atabeyarchaeia with the Asgardarchaeota phylum.

The type species for this taxonomic group were initially reconstructed by manual curation of Illumina short-read assemblies and then validated using both Nanopore and PacBio long reads. Phylogenetic analyses of this fully curated genome using several sets of marker genes placed the complete genomes in a monophyletic group within the Asgard clade as a sister group to Atabeyarchaeia. We performed phylogenetic analyses using concatenated marker sets of 47 archaeal clusters of orthologous genes (arCOGs) and 15 ribosomal protein (RP15) gene cluster, as well as the 16S rRNA gene. The new genomes share only <45% AAI when compared to other Asgard genomes, consistent with their assignment to a new phylum. Although our analyses provide evidence for distinction at the phylum level, we chose to adhere to the Genome Taxonomy Database (GTDB) for standardized microbial genome nomenclature. We establish a robust classification and propose Freyarchaeum as the type genus for the family Freyarchaeaceae, the order Freyarchaeales, the class Freyarchaeia with the Asgardarchaeota phylum. Based on protein content and compositional features, we infer that Freyarchaeia is a non-methanogenic acetogenic chemoheterotroph, with potential to grow lithoautotrophically by using H2 as an electron donor. This lineage was originally reconstructed and named from Guaymas Basin, located in the Gulf of California, México, and from Jinze Hot Spring (Yunnan, China). Subsequently, another group used the original data to recover similar genomes and referred to them as Jordarchaeia. Here, we retain the original nomenclature.

ased on protein content and compositional features, we infer that Atabeyarchaeia is a non-methanogenic acetogenic chemoheterotroph, with potential to grow lithoautotrophically by using H2 as an electron donor. Phylogenetic analyses using several sets of marker genes placed two complete genomes in a monophyletic group within the Asgard clade as a sister group to Freyarchaeia. We performed phylogenetic analyses using concatenated marker sets of 47 archaeal clusters of orthologous genes (arCOGs) and 15 ribosomal protein (RP15) gene cluster, as well as the 16S rRNA gene. The new genomes share only 40–45% AAI when compared to other Asgard genomes, consistent with their assignment to a new phylum. Although our analyses provide evidence for distinction at the phylum level, we chose to adhere to the Genome Taxonomy Database (GTDB) for standardized microbial genome nomenclature. We establish a robust classification and propose Atabeyarchaeum as the type genus for the family Atabeyarchaeaceae, the order Atabeyarchaeales, the class Atabeyarchaeia with the Asgardarchaeota phylum.

 Atabeyarchaeum deiteterre has a genome size of about 2.81 Mbp with an average GC% of 49.6%, recovered from California soil. Atabeyarchaeum 16S shares less than 75%  similarity with previously described Asgardarchaeota classes. A. deiteterre is an acetogenic chemoheterotroph like other Asgardarchaeota, including Freyarchaeia. Estimated completeness at 97.2%, contamination at 1.87%, and the presence of 16 S rRNA genes (1529 bp) and 40 tRNAs.

Freyarchaeum deiteterre has a genome size of about 3.58 Mbp with an average GC% of 40.7%, recovered from California soil. Freyarchaeum 16S shares less than 45%  similarity with previously described Asgardarchaeota classes. F. deiteterre is an acetogenic chemoheterotroph like other Asgardarchaeota, including other soil-associated Atabeyarchaeia. Estimated completeness at 98.13%, contamination at 4.21%, and the presence of 16 S rRNA genes (1264 bp) and 38 tRNAs.