Register list for Desulfocorpusculum asiaticum gen. nov. sp. nov. and their lineage

The class description is identical to that of the family Desulfocorpusculaceae.

The order description is identical to that of the family Desulfocorpusculaceae.

Desulfocorpusculaceae is a family of yet uncultured bacteria of the phylum Desulfobacterota. In addition to MAGs of the genus Desulfocorpusculum, this family also includes MAGs from hot springs of USA: Yellowstone (GCA_028275295.1), California (GCA_964514105.1); as well as from hot springs of Canada (GCA_964583655.1) and from Pacific Ocean hydrothermal fluid (GCA_002010915.1). This family is designated as f__JdFR-97 in GTDB R226.

Desulfocorpusculum is a genus of yet uncultured bacteria of the phylum Desulfobacterota. In addition to Desulfocorpusculum asiaticum U4-17, this genus also includes MAGs from hot springs of Costa Rica (GCA_037443445.1) and Canada (GCA_964414345.1). This genus is designated as g__JBBFLE01 in GTDB R226.

Desulfocorpusculum asiaticum U4-17 is presented by high-quality draft metagenome-assembled genome (MAG) of a member of the phylum Desulfobacterota, that was assembled from thermophilic microbial community from a hot stream formed by discharge of thermal artesian groundwater in Navoiy region (Republic of Uzbekistan).
The MAG has genes of the rod-shaped determining proteins, chemotaxis and the flagellar machinery required for motility. The genes essential for sporulation are absent. Encodes for the complete Embden-Meyerhof-Parnas and Wood-Ljungdahl pathways, non-oxidative branch of the pentose-phosphate pathway, pyruvate oxidation, the fatty acids β-oxidation and tricarboxylic acid cycle. Harbors genes involved in dissimilatory sulfate reduction (Sat-AprAB-DsrABC) including corresponding electron-transport complexes. Has only scant genes related to nitrogen metabolism. A complete electron transport chain is encoded including cytochromes oxidases as well as F-type H+/Na+- transporting ATPase.
The 16S rRNA-based analysis revealed that U4-17 is very distantly related to any of the known bacteria. The closest relative species were Geobacter benzoatilyticus Jerry-YXT (87.9% identity), Syntrophus aciditrophicus SBT (87.6%) and Desulfococcus multivorans 1be1T (87.4%), which belong to different classes of the phylum Desulfobacterota: Desulfuromonadia, Syntrophia and Desulfobacteria, respectively (Waite et al., 2020).
The results of phylogenomic reconstruction based on 120 single copy marker genes according to Parks et al., 2020 (bac120), but with much larger alignment length (20,331 aa), confirmed that the U4-17 with several MAGs form a phylogenetic cluster highly distant from other classes of Desulfobacterota.