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266


Confirmation of Candidatus Liberibacter asiaticus in Asian Citrus Psyllids and Detection of Asian Citrus Psyllids in Commercial Citrus in Georgia (U.S.A.)

Citation
Collins et al. (2024). Plant Disease
Names
Ca. Liberibacter asiaticus
Abstract
The Asian citrus psyllid (ACP) is the vector of Candidatus Liberibacter asiaticus (CLas), the causal agent of citrus greening or Huanglongbing (HLB), one of the most devastating citrus diseases worldwide. The citrus industry in Georgia (U.S.A.) is in the process of a rapid expansion, and based on experiences with HLB in Florida, there is great concern about the potential impacts of HLB on this emerging industry. Prior to 2023, ACP had been identified in residential citrus trees in isolated Geor

Multiplex qPCR for the detection of bacteria associated with huanglongbing 'Candidatus Liberibacter asiaticus', 'Ca. L. americanus' and 16Sr IX group phytoplasma

Citation
Martins et al. (2024). Plant Disease
Names
Ca. Phytoplasma Ca. Liberibacter asiaticus Liberibacter
Abstract
The occurrence of ‘Candidatus Liberibacter’ spp. and ‘Ca. Phytoplasma’ spp. associated with blotchy mottle symptoms poses challenges to huanglongbing (HLB) diagnosis using molecular techniques. The ability to detect multiple targets simultaneously and specifically is a key aspect met by qPCR. A set of primers and hydrolysis probes useful either in single or multiplex reactions for the detection and quantification of HLB-associated bacteria were developed. Sequences from conserved genes of the r

Development of a TaqMan Assay for the Detection of ‘Candidatus Phytoplasma brasiliense’ and Assessment by High-Resolution Melt Curve Analysis

Citation
Lane et al. (2024). Plant Disease 108 (10)
Names
Ca. Phytoplasma brasiliense
Abstract
‘Candidatus Phytoplasma brasiliense’ (CPB) is a phytoplasma originally discovered in South America and is known to infect a wide variety of economically important crops. It is most prevalent in Hibiscus spp., where it causes witches broom symptoms, and papaya, where it causes bunchy top. Recently, CPB was documented for the first time in North America in a new host, globe sedge. In this study, two quantitative PCR assays are developed: one using high-resolution melt curve analysis (HRMA) based

Natural Infection of Murraya paniculata and Murraya sumatrana with ‘Candidatus Liberibacter asiaticus’ in Java

Citation
Lestiyani et al. (2024). Plant Disease 108 (9)
Names
Ca. Liberibacter asiaticus
Abstract
The phloem-limited bacterium ‘Candidatus Liberibacter asiaticus’ (CLas) is the putative causal pathogen of the severe Asiatic form of huanglongbing (citrus greening) and is most commonly transmitted by the Asiatic citrus psyllid Diaphorina citri. CLas severely affects many Citrus species and hybrids and has been recorded in the Citrus relative, orange jasmine, Murraya paniculata (L.) Jack (syn. M. exotica L.). In this study, 13 accessions of three Murraya species (M. paniculata, M. sumatrana Ro

Putting ‘X’ into Context: The Diversity of ‘Candidatus Phytoplasma pruni’ Strains Associated with the Induction of X-Disease

Citation
Molnar et al. (2024). Plant Disease 108 (9)
Names
Ca. Phytoplasma pruni
Abstract
Recurrent epiphytotics of X-disease, caused by ‘Candidatus Phytoplasma pruni,’ have inflicted significant losses on commercial cherry and peach production across North America in the last century. During this period, there have been multiple studies reporting different disease phenotypes and, more recently, identifying different strains through sequencing core genes, but the symptoms have not, to date, been linked with genotype. Therefore, in this study we collected and assessed differing disea

Genomic analysis of "Candidatus Liberibacter africanus" strain from Zimbabwe reveals unique virulence and prophage characteristics compared to “Ca. Liberibacter asiaticus”

Citation
Zheng et al. (2024). Plant Disease
Names
“Liberibacter solanacearum” Ca. Liberibacter asiaticus Ca. Liberibacter africanus Liberibacter
Abstract
Citrus Huanglongbing (HLB) is caused by the phloem-limited α-proteobacterium “Candidatus Liberibacter spp.”, among which “Ca. Liberibacter africanus” (CLaf) have posed a significant threat to citrus production in Africa near a century. CLaf is closely related to the globally prevalent “Ca. Liberibacter asiaticus” (CLas), whereas little is known about the virulence of CLaf, primarily due to limited genome resources. In this study, we completed the whole-genome assembly and annotation of CLaf str