Levy, Amit


Publications
14

Transcriptomic profiling of ‘CandidatusLiberibacter asiaticus’ in different citrus tissues reveals novel insights into Huanglongbing pathogenesis

Citation
Lovelace et al. (2024).
Names
Ca. Liberibacter asiaticus
Abstract
ABSTRACT‘CandidatusLiberibacter asiaticus’ (Las) is a gram-negative bacterial pathogen associated with citrus huanglongbing (HLB) or greening disease. Las is transmitted by the Asian citrus psyllid (ACP) where it colonizes the phloem tissue, resulting in substantial economic losses to citrus industry worldwide. Despite extensive efforts, effective management strategies against HLB remain elusive, necessitating a deeper understanding of the pathogen’ s biology. Las undergoes cell-to-cell movement

Candidatus Liberibacter asiaticus accumulation in the phloem inhibits callose and reactive oxygen species

Citation
Bernardini et al. (2022). Plant Physiology 190 (2)
Names
Ca. Liberibacter asiaticus
Abstract
CLas inhibits callose deposition in the sieve pores and the accumulation of reactive oxygen species to favor its cell-to-cell movement.

Candidatus Liberibacter asiaticus reduces callose and reactive oxygen species production in the phloem

Citation
Bernardini et al. (2022).
Names
Ca. Liberibacter asiaticus
Abstract
AbstractHuanglongbing (HLB) causes significant economic loss in citrus production worldwide. HLB is caused by Candidatus Liberibacter asiaticus (CLas), a gram-negative bacterium which inhabits the phloem exclusively. CLas infection results in accumulation of callose and reactive oxygen species in the phloem of infected plants, but little is known about the specific processes that take place during infection because of the sparse distribution of bacteria and the inaccessibility of the phloem insi

Transcriptome Profiling of ‘Candidatus Liberibacter asiaticus’ in Citrus and Psyllids

Citation
De Francesco et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (Las) is an emergent bacterial pathogen that is associated with the devastating citrus huanglongbing (HLB). Vectored by the Asian citrus psyllid, Las colonizes the phloem tissue of citrus, causing severe damage to infected trees. So far, cultivating pure Las culture in axenic media has not been successful, and dual-transcriptome analyses aiming to profile gene expression in both Las and its hosts have a low coverage of the Las genome because of the low abunda

Intracellular Life Cycle of ‘Candidatus Liberibacter asiaticus’ Inside Psyllid Gut Cells

Citation
Chun-Yi et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (CLas), the devastating pathogen related to Huanglongbing (HLB), is a phloem-limited, fastidious, insect-borne bacterium. Rapid spread of HLB disease relies on CLas-efficient propagation in the vector, the Asian citrus psyllid Diaphorina citri, in a circulative manner. Understanding the intracellular lifecycle of CLas in psyllid midgut, the major organ for CLas transmission, is fundamental to improving current management strategies. Using a microscopic approa

Canopy health, but not Candidatus Liberibacter asiaticus Ct values, are correlated with fruit yield in Huanglongbing affected sweet orange trees

Citation
Levy et al. (2021).
Names
Ca. Liberibacter asiaticus
Abstract
AbstractIn Florida, almost all citrus trees are infected with Huanglongbing (HLB), caused by the gram-negative, intracellular phloem limited bacteria Candidatus liberibacter asiaticus (CLas). Distinguishing between the severely and mildly sick trees is important for managing the groves and testing new HLB therapies. A mildly sick tree is one that produces higher fruit yield, compared to a severely sick tree, but measuring yields is laborious and time consuming. Here we characterized HLB affected

Transcriptome profiling of Candidatus Liberibacter asiaticus in citrus and psyllids

Citation
De Francesco et al. (2021).
Names
Ca. Liberibacter asiaticus
Abstract
Candidatus Liberibacter asiaticus (Las) is an emergent bacterial pathogen that is associated with the devastating citrus Huanglongbing (HLB). Vectored by the Asian citrus psyllid, Las colonizes the phloem tissue of citrus, causing severe damage to infected trees. So far, cultivating pure Las culture in axenic media has not been successful and dual-transcriptome analyses aiming to profile gene expression in both Las and its host(s) have a low coverage of the Las genome due to the low abundance of

Plant hairy roots enable high throughput identification of antimicrobials against Candidatus Liberibacter spp

Citation
Irigoyen et al. (2020). Nature Communications 11 (1)
Names
Liberibacter
Abstract
AbstractA major bottleneck in identifying therapies to control citrus greening and other devastating plant diseases caused by fastidious pathogens is our inability to culture the pathogens in defined media or axenic cultures. As such, conventional approaches for antimicrobial evaluation (genetic or chemical) rely on time-consuming, low-throughput and inherently variable whole-plant assays. Here, we report that plant hairy roots support the growth of fastidious pathogens like Candidatus Liberibac