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cognitis nomina
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Authors Wen

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Wen, Aimin


Publications
4

CitationNamesAbstract
Genomes of ‘Candidatus Liberibacter solanacearum’ Haplotype A from New Zealand and the United States Suggest Significant Genome Plasticity in the Species Thompson et al. (2015). Phytopathology® 105 (7) “Liberibacter solanacearum”
Erratum to: Development of a PCR Assay for the Rapid Detection and Differentiation of ‘Candidatus Liberibacter solanacearum’ Haplotypes and Their Spatiotemporal Distribution in the United States Wen et al. (2013). American Journal of Potato Research 90 (3) “Liberibacter solanacearum”
Development of a PCR Assay for the Rapid Detection and Differentiation of ‘Candidatus Liberibacter solanacearum’ Haplotypes and Their Spatiotemporal Distribution in the United States Wen et al. (2013). American Journal of Potato Research 90 (3) “Liberibacter solanacearum”
Multiplex real-time PCR for detection, identification and quantification of ‘Candidatus Liberibacter solanacearum’ in potato plants with zebra chip Li et al. (2009). Journal of Microbiological Methods 78 (1) “Liberibacter solanacearum”

Genomes of ‘Candidatus Liberibacter solanacearum’ Haplotype A from New Zealand and the United States Suggest Significant Genome Plasticity in the Species
‘Candidatus Liberibacter solanacearum’ contains two solanaceous crop-infecting haplotypes, A and B. Two haplotype A draft genomes were assembled and compared with ZC1 (haplotype B), revealing inversion and relocation genomic rearrangements, numerous single-nucleotide polymorphisms, and differences in phage-related regions. Differences in prophage location and sequence were seen both within and between haplotype comparisons. OrthoMCL and BLAST analyses identified 46 putative coding sequences present in haplotype A that were not present in haplotype B. Thirty-eight of these loci were not found in sequences from other Liberibacter spp. Quantitative polymerase chain reaction (qPCR) assays designed to amplify sequences from 15 of these loci were screened against a panel of ‘Ca. L. solanacearum’-positive samples to investigate genetic diversity. Seven of the assays demonstrated within-haplotype diversity; five failed to amplify loci in at least one haplotype A sample while three assays produced amplicons from some haplotype B samples. Eight of the loci assays showed consistent A–B differentiation. Differences in genome arrangements, prophage, and qPCR results suggesting locus diversity within the haplotypes provide more evidence for genetic complexity in this emerging bacterial species.
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