Genome sc|0002756

Genomics

Accession

NCBI Assembly:GCA_016715285.1

Type

Metagenome-Assembled Genome (MAG)

Estimated Quality Metrics

• Completeness: 96.22%
• Contamination: 2.16%
• Quality: 85.42

Ribosomal and transfer RNA genes

• 1 16S rRNA (up to 100.0%)
• 1 23S rRNA (up to 100.0%)
• tRNAs for 19 amino acids

Sequencing depth

13.3578 ×

Source

• BioSample: SAMN16426287

Other features

• G+C Content: 66.66%
• Coding Density: 90.2%
• Codon Table: 11
• N50: 460,571 bp
• Contigs: 12
• Largest Contig: 685,773 bp
• Assembly Length: 3,282,635 bp
• Ambiguous Assembly Fraction: 0.0%

Submitter comments

The binning in this project followed the mmlong v0.1.2 hybrid-metaflowpipeline after the assembly step. Metagenome contigs were translated into proteins using FragGeneScan v1.31, annotated taxonomically using Kaiju v1.6.0 against the proGenomes database (2017-05-16). DASTool v1.1.1 --search_engine diamond was used to dereplicate and select for the best representative bin. The dereplicated bins in the analysis were checked for completeness and contamination using CheckM --lineage_wf v1.0.11.
Genome taxonomy was determined using GTDB-Tk v1.0.2 and the refseq release 89 (2019-06-19) database, and the dependencies pplacer v1.1, FastANI v1.2, Prodigal v2.6.2, FastTree 2 v1.2, and HMMER v3.1b2. 

Automated checks

Complete

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Last modified 26 days ago

Sample Metadata

BioSample entries

• BioSample: SAMN16426287

Date

Collection Date: 2018-08-29

Location

Lat Lon: 56.742252 N 10.114475 E

Toponym

Geo Loc Name: Denmark: Mariagerfjord

Environment

Sample Type: metagenomic assembly

Isolation Source: activated sludge from Mari_18-Q3-R65-66

Env Broad Scale: aquatic biome [ENVO:00002030]

Env Local Scale: Waste Water [ENVO:00002001]

Env Medium: Activated Sludge [ENVO:00002046]

Other

Not detected

Package

Ncbi Submission Package: MIMAG.wastewater.5.0

Biosamplemodel: MIMAG.wastewater

Additional Accessions

SAMN14825711

All retrieved samples

• SAMN16426287: MIMAG Metagenome-assembled Genome sample from Actinomycetales bacterium

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Metadata retrieved 26 days ago