Hybrid assembly was performed by Unicyler v0.4.8 (Wick et al. 2017) in hybrid using the recruited short-read pairs and long-read data sets as input. The A. flamelloides BUSSELTON2 reassembly produced a unambiguous circular assembly of 4,968,664 bp. The assembled symbiont genomes were annotated by Rapid Annotation using Subsystem Technology 2.0 RAST: (Aziz et al. 2008).