Environmental genomic DNA of enrichment samples were duplexed and sequenced within the Illumina platform from Beijing Biomark Biotechnology, which were then assembled into longer contiguous sequences known as contigs using SPAdes (ver. 3.15.5) (Harrison, Strulo, 2000). The contigs were grouped into bins based on their similarities, utilizing characteristics such as coverage depth and sequence composition, and the quality of the recovered bins was assessed using tools like CheckM or QUAST to evaluate completeness and contamination levels. Medium to high-quality metagenome-assembled genomes (MAGs) were classified using the GTDB and annotated to predict their functional capabilities via databases such as KEGG or COG.