Genome quality including completeness, contamination was evaluated using CheckM. 16S and 23S rRNA sequences from MAGs were called using barrnap. tRNAs were detected using tRNAscan-SE.
The complete 1.54 megabase pair (Mbp) circular genome of strain LWZ-6 (DNA G+C content of 54.42 mol%; 1606 open reading frames) was obtained by reassembly of Nanopore and Illumina sequencing data. Strain LWZ-6 employs obligate H2-dependent methylotrophic methanogenesis (Methanol and monomethylamine).