Genomic annotation was carried out using Prokka. Genomic islands were predicted using IslandViewer 4. Genomic relatedness with type strains of members of the Harveyi clade was determined using OrthoANIu algorithm (http://www.ezbiocloud.net/tools/ani). Digital DDH values were calculated using genome–genome distance calculator (GGDC 2.1) applying Formula 2 (identities/HSP length). Average amino acid index (AAI) between SBOTS_Iso1 with type strains of Harveyi clade was determined using AAI-profler (http://ekhidna2.bioce nter.helsinki.f/AAI/). In silico phenotyping was performed using Traitar (https://github.com/hzi-bifo/traitar). Genome sequence of SBOTS_Iso1 and closest relative, V.alginolyticus ATCC17749T was aligned in progressiveMauve (http://gel.ahabs.wisc.edu/mauve). The genome of SBOTS_Iso1 was aligned into circular map using CGView server (http://stothard.afns.ualberta.ca/cgibin/cgview_server/cgview_server.pl).