Phytopathology®


Publications
80

Heat Treatment Eliminates ‘Candidatus Liberibacter asiaticus’ from Infected Citrus Trees Under Controlled Conditions

Citation
Hoffman et al. (2013). Phytopathology® 103 (1)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB) is one of the most destructive diseases of citrus worldwide. The three known causal agents of HLB are species of α-proteobacteria: ‘Candidatus Liberibacter asiaticus’, ‘Ca. L. africanus’, and ‘Ca. L. americanus’. Previous studies have found distinct variations in temperature sensitivity and tolerance among these species. Here, we describe the use of controlled heat treatments to cure HLB caused by ‘Ca. L. asiaticus’, the most prevalent and heat-tolerant species. Using temper

Transmission Efficiency of ‘Candidatus Liberibacter solanacearum’ and Potato Zebra Chip Disease Progress in Relation to Pathogen Titer, Vector Numbers, and Feeding Sites

Citation
Rashed et al. (2012). Phytopathology® 102 (11)
Names
“Liberibacter solanacearum”
Abstract
With diseases caused by vector-borne plant pathogens, acquisition and inoculation are two primary stages of the transmission, which can determine vector efficiency in spreading the pathogen. The present study was initiated to quantify acquisition and inoculation successes of ‘Candidatus Liberibacter solanacearum’, the etiological agent of zebra chip disease of potato, by its psyllid vector, Bactericera cockerelli (Hemiptera: Triozidae). Acquisition success was evaluated in relation to feeding s

Development of a Loop-Mediated Isothermal Amplification Procedure as a Sensitive and Rapid Method for Detection of ‘Candidatus Liberibacter solanacearum’ in Potatoes and Psyllids

Citation
Ravindran et al. (2012). Phytopathology® 102 (9)
Names
“Liberibacter solanacearum”
Abstract
This study reports the development of a loop-mediated isothermal amplification procedure (LAMP) for polymerase chain reaction (PCR)-based detection of ‘Candidatus Liberibacter solanacearum’, the bacterial causal agent of potato zebra chip (ZC) disease. The 16S rDNA gene of ‘Ca. Liberibacter solanacearum’ was used to design a set of six primers for LAMP PCR detection of the bacterial pathogen in potato plants and the psyllid vector. The advantage of the LAMP method is that it does not require a

Effect of ‘Candidatus Liberibacter solanacearum’ on Fitness of Its Insect Vector, Bactericera cockerelli (Hemiptera: Triozidae), on Tomato

Citation
Nachappa et al. (2012). Phytopathology® 102 (1)
Names
“Liberibacter solanacearum”
Abstract
The potato/tomato psyllid, Bactericera cockerelli transmits the bacterium ‘Candidatus Liberibacter solanacearum’, also known as ‘Ca. L. psyllaurous’, which causes zebra chip disease in solanaceous crops. There have been no studies addressing the effect of the bacterial plant pathogen on the biology of its insect vector. We examined several life-history traits, including 7-day fecundity, hatching percentage, incubation time, nymphal survival percentage, nymphal development time, total developmen

Colonization of Dodder, Cuscuta indecora, by ‘Candidatus Liberibacter asiaticus’ and ‘Ca. L. americanus’

Citation
Hartung et al. (2010). Phytopathology® 100 (8)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing, or citrus greening, threatens the global citrus industry. The presumptive pathogens, ‘Candidatus Liberibacter asiaticus’ and ‘Ca. L. americanus’ can be transferred from citrus to more easily studied experimental hosts by using holoparasitic dodder plants. However, the interaction between ‘Candidatus Liberibacter’ spp. and the dodder has not been studied. We combined quantitative polymerase chain reaction with electron microscopy to show that only 65% of tendrils of Cuscuta indeco

Screening Molecules for Control of Citrus Huanglongbing Using an Optimized Regeneration System for ‘Candidatus Liberibacter asiaticus’-Infected Periwinkle (Catharanthus roseus) Cuttings

Citation
Zhang et al. (2010). Phytopathology® 100 (3)
Names
Ca. Liberibacter asiaticus
Abstract
Citrus huanglongbing is one of the most destructive diseases of citrus worldwide. The disease is associated with three different species of ‘Candidatus Liberibacter’, of which ‘Ca. L. asiaticus’ is the most widely distributed. An optimized system using ‘Ca. L. asiaticus’-infected periwinkle cuttings was developed to screen chemical compounds effective for controlling the bacterial population while simultaneously assessing their phytotoxicity. The optimal regeneration conditions were determined

Graft Transmission Efficiencies and Multiplication of ‘Candidatus Liberibacter americanus’ and ‘Ca. Liberibacter asiaticus’ in Citrus Plants

Citation
Lopes et al. (2009). Phytopathology® 99 (3)
Names
Ca. Liberibacter americanus Ca. Liberibacter asiaticus
Abstract
In Brazil ‘Candidatus Liberibacter asiaticus’ and ‘Ca. L. americanus’ cause huanglongbing (also known as greening), the most destructive citrus disease. A shift in pathogen prevalence was observed over time, with a disproportional increase in ‘Ca. L. asiaticus’ occurrence. Graft transmission experiments were used for a comparative study of both species using budsticks from symptomatic branches of field-affected trees as inoculum. The plants were inoculated with ‘Ca. L. asiaticus’ or ‘Ca. L. ame

A Phytoplasma Related to ‘Candidatus Phytoplasma asteris’ Detected in Citrus Showing Huanglongbing (Yellow Shoot Disease) Symptoms in Guangdong, P. R. China

Citation
Chen et al. (2009). Phytopathology® 99 (3)
Names
Ca. Phytoplasma asteris
Abstract
Citrus huanglongbing (HLB) or yellow shoot disease (i.e., greening disease) is highly destructive to citrus production worldwide. Understanding the etiology of HLB is critical for managing the disease. HLB is currently associated with infection by ‘Candidatus Liberibacter spp.’ around the world, including China. However, Koch's postulates have not been fulfilled. In addition, other plant pathogens also may be involved in HLB. In a survey performed in Guangdong Province, P. R. China in 2006 and

Quantitative Distribution of ‘Candidatus Liberibacter asiaticus’ in Citrus Plants with Citrus Huanglongbing

Citation
Li et al. (2009). Phytopathology® 99 (2)
Names
Ca. Liberibacter asiaticus
Abstract
Citrus huanglongbing (HLB), or greening disease, is strongly associated with any of three nonculturable gram-negative bacteria belonging to ‘Candidatus Liberibacter spp.’ ‘Ca. Liberibacter spp.’ are transmitted by citrus psyllids to all commercial cultivars of citrus. The diseases can be lethal to citrus and have recently become widespread in both São Paulo, Brazil, and Florida, United States, the locations of the largest citrus industries in the world. Asiatic HLB, the form of the disease foun

Detection of ‘Candidatus Liberibacter asiaticus’ in Diaphorina citri and Its Importance in the Management of Citrus Huanglongbing in Florida

Citation
Manjunath et al. (2008). Phytopathology® 98 (4)
Names
Ca. Liberibacter asiaticus
Abstract
Citrus huanglongbing (HLB or citrus greening), is a highly destructive disease that has been spreading in both Florida and Brazil. Its psyllid vector, Diaphorina citri Kuwayama, has spread to Texas and Mexico, thus threatening the future of citrus production elsewhere in mainland North America. Even though sensitive diagnostic methods have been developed for detection of the causal organisms, Candidatus Liberibacter spp., the pathogen cannot be detected consistently in plants until symptoms dev