Register list for Patescibacterium danicum gen. nov. sp. nov. and their lineage

The phylum represents a monophyletic lineage based on the concatenated phylogeny of 120 protein markers and contains the class Patescibacteriia. This phylum is characterized by members with a low genomic GC content (33.6 ± 6.7%), reduced genome sizes (919.3 kbp ± 256 kbp) and limited metabolic capabilities, that are likely to rely on symbiotic partners for survival. The type genus is Patescibacterium.

The class represents a monophyletic lineage based on the concatenated phylogeny of 120 protein markers and contains the order Patescibacteriales. The description of the class is the same as for the order Patescibacteriales. The type genus is Patescibacterium.

The order represents a monophyletic lineage based on the concatenated phylogeny of 120 protein markers and contains the family Patescibacteriaceae. The description of the order is the same as for the family Patescibacteriaceae. The type genus is Patescibacterium.

The family represents a monophyletic lineage based on the concatenated phylogeny of 120 protein markers and contains the genus Patescibacterium. The description of the family is the same as for its type genus Patescibacterium.

The description of the genus is based on the single species which is characterized by a small genome size below 1 Mbp and inferred to lack nucleotide and amino acid synthesis, which is compensated by encoded DNA uptake and peptide cleaving abilities. The lack of a pentose phosphate pathway (PPP) and a complete tricarboxylic acid (TCA) cycle leaves anaerobic glycolysis, albeit without the key enzyme phosphofructokinase-1 (PFK-1), as a means of energy conservation. 
The type species of the genus is Patescibacterium danicum, obtained from the Mariagerfjord WWTP in Denmark.

The species is represented by the metagenome-assembled genome (MAG) sequence ABY1, available in GTDB as GCA_016699775.1, obtained from metagenomic sequences from Mariagerfjord WWTP; Denmark. ABY1 represents a complete, single-contig genome of 938,851 bp, with a GC content of 35.4%, a coding density of 88.17% and a protein count of 890. ABY1 encodes one 5S, 16S, and 23S ribosomal RNA gene and 41 tRNAs.