Cloacimonas acidaminivorans sp. nov. and Cloacimonas gen. nov.

The description is the same as that for Cloacimonas acidaminivorans.

The complete genome of this species has been reconstructed following the metagenomic sequencing of the sludge sample obtained from the anaerobic mesophilic digester (7,000 m3) at the Evry wastewater treatment plant (250,000 population equivalents), located about 35 km south of Paris, France (48°37′ 33.65"N, 2°27′53.34"E). The genome of this species is represented by a single circular chromosome consisting of 2,246,820 nucleotides with a GC content of 37.9%. The presence of the enzymes involved in the biosynthesis of lipopolysaccharides indicates that is a gram-negative bacterium, which is supported by the detection of the typical signature of gram-negative bacteria found in the dnaK gene. The in silico proteome analysis suggests that this species could grow on a medium that includes glucose, alanine, asparagine, aspartate, glutamate, histidine, lysine, proline, serine, and certain aliphatic carboxylic acids (succinate, lactate, and acetate). The species is inferred to be adapted to an anaerobic lifestyle and also may enable to survive under minimal oxygen concentrations. In silico proteome analysis indicated that this species might derive most of its carbon and energy from the fermentation of amino acids and it is probably a syntrophic bacterium.