Abstract
With the aim of describing the human microbiota by the means of culture methods, culturomics was developed in order to target previously un‐isolated bacterial species and describe it via the taxono‐genomics approach. While performing a descriptive study of the human gut microbiota of the pygmy people, strain Marseille‐P4678
T
has been isolated from a stool sample of a healthy 39‐year‐old pygmy male. Cells of this strain were Gram‐positive cocci, spore‐forming, non‐motile, catalase‐positive and oxidase‐negative, and grow optimally at 37°C under anaerobic conditions. Its 16S
rRNA
gene sequence exhibited 89.69% of sequence similarity with
Parvimonas micra
strain 3119
B
T
(
NR
036934.1), its phylogenetically closest species with standing in nomenclature. The genome of strain Marseille‐P4678
T
is 2,083,161 long with 28.26 mol% of G+C content. Based on its phenotypic, biochemical, genotypic and proteomic profile, this bacterium was classified as a new bacterial genus and species
Miniphocibacter massiliensis
gen. nov., sp. nov. with the type strain Marseille‐P4678
T
.