Merkel, Alexander Y.


Publications
15

A Simple and Rapid System for Proteomic Analysis of the Archaeon Candidatus Vulcanisaeta moutnovskia

Citation
Chernyh et al. (2020).
Names
Ca. Vulcanisaeta moutnovskia
Abstract
Abstract This protocol describes a rapid protein extraction method for the archaeon Candidatus Vulcanisaeta moutnovskia, which can be also implemented for other archaea. The utilization of two different methods for protein extraction constitute the main step of the protocol. Method I involves the extraction with a multi-chaotropic lysis buffer containing a non-denaturing zwitterionic detergent, most efficient for extracting cytosolic proteins. Method II involves a denaturing anionic dete

Characterization of<scp>M</scp>elioribacter roseusgen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class<scp>I</scp>gnavibacteria, and a proposal of a novel bacterial phylum<scp>I</scp>gnavibacteriae

Citation
Podosokorskaya et al. (2013). Environmental Microbiology 15 (6)
Names
Ignavibacteriota
Abstract
SummaryA novel moderately thermophilic, facultatively anaerobic chemoorganotrophic bacterium strainP3M‐2Twas isolated from a microbial mat developing on the wooden surface of a chute under the flow of hot water (46°C) coming out of a 2775‐m‐deep oil exploration well (Tomsk region,Russia). StrainP3M‐2Tis a moderate thermophile and facultative anaerobe growing on mono‐, di‐ or polysaccharides by aerobic respiration, fermentation or by reducing diverse electron acceptors [nitrite,Fe(III),As(V)]. It