Brlansky, Ron H.

Candidatus Liberibacter asiaticus (CLas) is one of the putative causal agents of huanglongbing, which is a serious disease in citrus production. The pathogen is transmitted by Diaphorina citri Kuwayama (Hemiptera: Psyllidae). As an observational study, six groves in central Florida and one grove at the southern tip of Florida were sampled monthly from January 2008 through February 2012 (50 months). The collected psyllids were sorted by sex and abdominal color. Disease prevalence in adults peaked in November, with a minor peak in February. Gray/brown females had the highest prevalence, and blue/green individuals of either sex had the lowest prevalence. CLas prevalence in blue/green females was highly correlated with the prevalence in other sexes and colors. Thus, the underlying causes for seasonal fluctuations in prevalence operated in a similar fashion for all psyllids. The pattern was caused by larger nymphs displacing smaller ones from the optimal feeding sites and immunological robustness in different sex–color morphotypes. Alternative hypotheses were also considered. Improving our understanding of biological interactions and how to sample them will improve management decisions. We agree with other authors that psyllid management is critical year-round.

Although there are no known sources of genetic resistance, some Citrus spp. are reportedly tolerant to huanglongbing (HLB), presumably caused by ‘Candidatus Liberibacter asiaticus’. Time-course transcriptional analysis of tolerant rough lemon (Citrus jambhiri) and susceptible sweet orange (C. sinensis) in response to ‘Ca. L. asiaticus’ infection showed more genes differentially expressed in HLB-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, possibly a critical factor underlying differences in sensitivity to ‘Ca. L. asiaticus’. Pathway analysis revealed that stress responses were distinctively modulated in rough lemon and sweet orange. Although microscopic changes (e.g., callose deposition in sieve elements and phloem cell collapse) were found in both infected species, remarkably, phloem transport activity in midribs of source leaves in rough lemon was much less affected by HLB than in sweet orange. The difference in phloem cell transport activities is also implicated in the differential sensitivity to HLB between the two species. The results potentially lead to identification of key genes and the genetic mechanism in rough lemon to restrain disease development and maintain (or recover) phloem transport activity. These potential candidate genes may be used for improving citrus tolerance (or even resistance) to HLB by genetic engineering.