Keywords: GSC:MIxS;MIMAG:6.0

collection_date

2019-10-26

INSDC secondary accession

SRS20635743

organism

Asgard group archaeon SRVP18_Atabeyarchaeia-1

env_broad_scale

temperate woodland biome [ENVO:01000221]

metagenomic

TRUE

sample_type

metagenomic assembly

environmental-sample

TRUE

env_local_scale

lake bottom mud [ENVO:00005797]

metagenome-source

freshwater sediment metagenome

isolate

SRVP18_Atabeyarchaeia-1

NCBI submission package

MIMAG.soil.6.0

depth

100cm

elev

200m

geo_loc_name

USA: California, Lake County

env_medium

lake bottom mud [ENVO:00005797]

lat_lon

38.41 N 122.31 W

isolation_source

sediment of a seasonal wetland

sample derived-from

SAMN21157288

BioSampleModel

MIMAG.soil

ENA-FIRST-PUBLIC

2024-02-27

ENA-LAST-UPDATE

2024-03-12

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="SRX23813131" alias="SRVP18_Atabeyarchaeia-1" center_name="SUB14273690" broker_name="NCBI">
  <IDENTIFIERS>
    <PRIMARY_ID>SRX23813131</PRIMARY_ID>
    <SUBMITTER_ID namespace="SUB14273690">SRVP18_Atabeyarchaeia-1</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>SRVP18_Atabeyarchaeia-1</TITLE>
  <STUDY_REF accession="SRP492896">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP492896</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject">PRJNA1050611</EXTERNAL_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION>Library preparation was performed using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina. Final sequencing-ready libraries were visualized using the Agilent 2100 Bioanalyzer before pooling, and the final pooled library was quantified through qPCR using the KAPA Library Quantification Kit for Illumina. Sequencing was performed using Illumina NextSeq 1000/2000 150 paired-end sequencing lanes with 10% PhiX spike-in controls. Post-sequencing bcl files were converted to demultiplexed fastq files per the original sample count with Illuminas bcl2fastq v2.20 software.</DESIGN_DESCRIPTION>
    <SAMPLE_DESCRIPTOR accession="SRS20635743">
      <IDENTIFIERS>
        <PRIMARY_ID>SRS20635743</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioSample">SAMN40176692</EXTERNAL_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>SRVP18_Atabeyarchaeia-1</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <PAIRED/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <ILLUMINA>
      <INSTRUMENT_MODEL>Illumina NovaSeq X</INSTRUMENT_MODEL>
    </ILLUMINA>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>SRS20635743</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>SRA1815393</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX23813131&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX23813131&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
</EXPERIMENT>
</EXPERIMENT_SET>