The quality metrics of the genome were assessed using multiple tools to ensure its reliability and integrity. The GC content was calculated using FastQC, a widely utilized tool for evaluating sequencing data, which provided a clear visualization of nucleotide composition and helped identify potential anomalies such as contamination or sequencing biases. Genome completeness and contamination were evaluated using CheckM, which employs lineage-specific marker genes to provide accurate estimates of these metrics, ensuring a robust assessment of the genome assembly’s quality. Additionally, the presence and integrity of the 16S and 23S rRNA genes were analyzed using Barrnap (BAsic Rapid Ribosomal RNA Predictor), a tool designed to predict ribosomal RNA sequences from genome assemblies, offering insights into the genome’s functional and phylogenetic quality.