Keywords: GSC:MIxS;MIGS:6.0

BioSample Accession(s)

SAMN47585658, SRS25163752

strain

MBAC-LM

collection_date

2017-06

isol_growth_condt

PMID: 10758888, https://mediadive.dsmz.de/medium/1523

ref_biomaterial

unpublished

INSDC secondary accession

SRS25163752

organism

Methanobacterium sp. MBAC-LM

env_broad_scale

desert area [ENVO:00000097]

env_local_scale

sandy desert [ENVO:00000172]

NCBI submission package

MIGS.ba.soil.6.0

depth

5 cm

elev

524 m

num_replicons

1

geo_loc_name

Israel: Midreshet Ben-Gurion

env_medium

biocrust [ENVO:01000909]

lat_lon

30.8410 N 34.7614 E

BioSampleModel

MIGS/MIMS/MIMARKS.soil

ENA-FIRST-PUBLIC

2025-03-27

ENA-LAST-UPDATE

2025-05-27

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="SRX28941296" alias="MBAC-LM-RAW" center_name="SUB15345941" broker_name="NCBI">
  <IDENTIFIERS>
    <PRIMARY_ID>SRX28941296</PRIMARY_ID>
    <SUBMITTER_ID namespace="SUB15345941">MBAC-LM-RAW</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>PacBio-HiFi of Methanobacterium: desert biocrust</TITLE>
  <STUDY_REF accession="SRP576492">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP576492</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject">PRJNA1242298</EXTERNAL_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION>HiFi sequencing using PacBio Sequel II platform. Genomic DNA was randomly sheared, followed by SMRTbell library preparation and size selection.</DESIGN_DESCRIPTION>
    <SAMPLE_DESCRIPTOR accession="SRS25163752">
      <IDENTIFIERS>
        <PRIMARY_ID>SRS25163752</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioSample">SAMN47585658</EXTERNAL_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>MBAC-LM-RAW</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>GENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <SINGLE/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <PACBIO_SMRT>
      <INSTRUMENT_MODEL>Sequel II</INSTRUMENT_MODEL>
    </PACBIO_SMRT>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>SRS25163752</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>SRA2137106</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX28941296&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX28941296&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
  <EXPERIMENT_ATTRIBUTES>
    <EXPERIMENT_ATTRIBUTE>
      <TAG>ENA-STATUS-ID</TAG>
      <VALUE>4</VALUE>
    </EXPERIMENT_ATTRIBUTE>
  </EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>