Bunger Hills soil from Helipad Zone, sample BH-09 (shotgun metagenome)

BioSample Accession(s)

ERS25059173, SAMEA118572240

ENA-CHECKLIST

ERC000022

altitude

3.19

broad-scale environmental context

polar desert biome

collection date

2023-02-06

depth

50

elevation

3.19

environmental medium

soil

geographic location (country and/or sea)

Antarctica

geographic location (latitude)

-66.25013121

geographic location (longitude)

100.6022538

geographic location (region and locality)

Wilkes Land, Bunger Hills, Edgeworth David Field Camp

local environmental context

ground soil

nucleic acid extraction

FASTDNA� SPIN Kit for Soil (MP Biomedicals, USA)

pH

7.1

pH method

APHA 4500 H+ � B (1:5)

project name

Bunger Hills metagenomics

salinity method

APHA 2510 B

sample storage conditions

-80

sample volume or weight for DNA extraction

0.3

total organic carbon

3.4

total organic carbon method

ALS standard method EP003

water content

5.2

water content method

Dried @ 105-110�C

Bunger Hills soil from Helipad Zone, sample BH-10 (shotgun metagenome)

BioSample Accession(s)

ERS25059174, SAMEA118572241

ENA-CHECKLIST

ERC000022

altitude

3.19

broad-scale environmental context

polar desert biome

collection date

2023-02-06

depth

50

elevation

3.19

environmental medium

soil

geographic location (country and/or sea)

Antarctica

geographic location (latitude)

-66.25013292

geographic location (longitude)

100.6022595

geographic location (region and locality)

Wilkes Land, Bunger Hills, Edgeworth David Field Camp

local environmental context

ground soil

nucleic acid extraction

FASTDNA� SPIN Kit for Soil (MP Biomedicals, USA)

pH

6.8

pH method

APHA 4500 H+ � B (1:5)

project name

Bunger Hills metagenomics

salinity method

APHA 2510 B

sample storage conditions

-80

sample volume or weight for DNA extraction

0.3

total organic carbon

4.3

total organic carbon method

ALS standard method EP003

water content

8

water content method

Dried @ 105-110�C

Bunger Hills soil from Helipad Zone, sample BH-11 (shotgun metagenome)

BioSample Accession(s)

ERS25059175, SAMEA118572242

ENA-CHECKLIST

ERC000022

altitude

2.94

broad-scale environmental context

polar desert biome

collection date

2023-02-06

depth

200

elevation

2.94

environmental medium

soil

geographic location (country and/or sea)

Antarctica

geographic location (latitude)

-66.25013015

geographic location (longitude)

100.6022547

geographic location (region and locality)

Wilkes Land, Bunger Hills, Edgeworth David Field Camp

local environmental context

ground soil

nucleic acid extraction

FASTDNA� SPIN Kit for Soil (MP Biomedicals, USA)

pH

6.5

pH method

APHA 4500 H+ � B (1:5)

project name

Bunger Hills metagenomics

salinity method

APHA 2510 B

sample storage conditions

-80

sample volume or weight for DNA extraction

0.3

total organic carbon

1.3

total organic carbon method

ALS standard method EP003

water content

12

water content method

Dried @ 105-110�C

Bunger Hills soil from Helipad Transect, sample BH-18 (shotgun metagenome)

BioSample Accession(s)

ERS25059176, SAMEA118572243

ENA-CHECKLIST

ERC000022

altitude

3.44

broad-scale environmental context

polar desert biome

collection date

2023-02-06

depth

50

elevation

3.44

environmental medium

soil

geographic location (country and/or sea)

Antarctica

geographic location (latitude)

-66.25031324

geographic location (longitude)

100.6018866

geographic location (region and locality)

Wilkes Land, Bunger Hills, Edgeworth David Field Camp

local environmental context

ground soil

nucleic acid extraction

FASTDNA� SPIN Kit for Soil (MP Biomedicals, USA)

pH

8.5

pH method

APHA 4500 H+ � B (1:5)

project name

Bunger Hills metagenomics

salinity method

APHA 2510 B

sample storage conditions

-80

sample volume or weight for DNA extraction

0.3

total organic carbon

2.5

total organic carbon method

ALS standard method EP003

water content

7.5

water content method

Dried @ 105-110�C

Bunger Hills soil from Background Transect, sample BH-20 (shotgun metagenome)

BioSample Accession(s)

ERS25059177, SAMEA118572244

ENA-CHECKLIST

ERC000022

altitude

5.79

broad-scale environmental context

polar desert biome

collection date

2023-02-06

depth

50

elevation

5.79

environmental medium

soil

geographic location (country and/or sea)

Antarctica

geographic location (latitude)

-66.2502947

geographic location (longitude)

100.6042694

geographic location (region and locality)

Wilkes Land, Bunger Hills, Edgeworth David Field Camp

local environmental context

ground soil

nucleic acid extraction

FASTDNA� SPIN Kit for Soil (MP Biomedicals, USA)

pH

8.8

pH method

APHA 4500 H+ � B (1:5)

project name

Bunger Hills metagenomics

salinity method

APHA 2510 B

sample storage conditions

-80

sample volume or weight for DNA extraction

0.3

total organic carbon

0.9

total organic carbon method

ALS standard method EP003

water content

3.4

water content method

Dried @ 105-110�C

Bunger Hills soil from Background Transect, sample BH-23 (shotgun metagenome)

BioSample Accession(s)

ERS25059178, SAMEA118572245

ENA-CHECKLIST

ERC000022

altitude

5.09

broad-scale environmental context

polar desert biome

collection date

2023-02-06

depth

50

elevation

5.09

environmental medium

soil

geographic location (country and/or sea)

Antarctica

geographic location (latitude)

-66.2507534

geographic location (longitude)

100.6030817

geographic location (region and locality)

Wilkes Land, Bunger Hills, Edgeworth David Field Camp

local environmental context

ground soil

nucleic acid extraction

FASTDNA� SPIN Kit for Soil (MP Biomedicals, USA)

pH

10

pH method

APHA 4500 H+ � B (1:5)

project name

Bunger Hills metagenomics

salinity method

APHA 2510 B

sample storage conditions

-80

sample volume or weight for DNA extraction

0.3

total organic carbon

1.5

total organic carbon method

ALS standard method EP003

water content

8

water content method

Dried @ 105-110�C

Bunger Hills soil from Background Transect, sample BH-24 (shotgun metagenome)

BioSample Accession(s)

ERS25059179, SAMEA118572246

ENA-CHECKLIST

ERC000022

altitude

6.19

broad-scale environmental context

polar desert biome

collection date

2023-02-06

depth

50

elevation

6.19

environmental medium

soil

geographic location (country and/or sea)

Antarctica

geographic location (latitude)

-66.2509134

geographic location (longitude)

100.6026622

geographic location (region and locality)

Wilkes Land, Bunger Hills, Edgeworth David Field Camp

local environmental context

ground soil

nucleic acid extraction

FASTDNA� SPIN Kit for Soil (MP Biomedicals, USA)

pH

9

pH method

APHA 4500 H+ � B (1:5)

project name

Bunger Hills metagenomics

salinity method

APHA 2510 B

sample storage conditions

-80

sample volume or weight for DNA extraction

0.3

total organic carbon

1.3

total organic carbon method

ALS standard method EP003

water content

1.4

water content method

Dried @ 105-110�C

Metadata as EBI XML

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Metadata as EBI XML

<EXPERIMENT_SET>
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  <DESIGN>
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Metadata as EBI XML

<EXPERIMENT_SET>
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  <TITLE>Illumina NovaSeq X Plus paired end sequencing</TITLE>
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  <DESIGN>
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      <IDENTIFIERS>
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    <LIBRARY_DESCRIPTOR>
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  <EXPERIMENT_LINKS>
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Metadata as EBI XML

<EXPERIMENT_SET>
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Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="ERX14538610" alias="ena-EXPERIMENT-TAB-19-06-2025-01:56:43:723-9114" center_name="THE UNIVERSITY OF NEW SOUTH WALES">
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Metadata as EBI XML

<EXPERIMENT_SET>
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  <DESIGN>
    <DESIGN_DESCRIPTION/>
    <SAMPLE_DESCRIPTOR accession="ERS25059178">
      <IDENTIFIERS>
        <PRIMARY_ID>ERS25059178</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioSample">SAMEA118572245</EXTERNAL_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME/>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <PAIRED/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <ILLUMINA>
      <INSTRUMENT_MODEL>Illumina NovaSeq X Plus</INSTRUMENT_MODEL>
    </ILLUMINA>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>ERS25059178</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>ERA33359780</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX14538611&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX14538611&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
  <EXPERIMENT_ATTRIBUTES>
    <EXPERIMENT_ATTRIBUTE>
      <TAG>ENA-STATUS-ID</TAG>
      <VALUE>4</VALUE>
    </EXPERIMENT_ATTRIBUTE>
  </EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="ERX14538612" alias="ena-EXPERIMENT-TAB-19-06-2025-01:56:43:723-9118" center_name="THE UNIVERSITY OF NEW SOUTH WALES">
  <IDENTIFIERS>
    <PRIMARY_ID>ERX14538612</PRIMARY_ID>
    <SUBMITTER_ID namespace="THE UNIVERSITY OF NEW SOUTH WALES">ena-EXPERIMENT-TAB-19-06-2025-01:56:43:723-9118</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>Illumina NovaSeq X Plus paired end sequencing</TITLE>
  <STUDY_REF accession="ERP162030">
    <IDENTIFIERS>
      <PRIMARY_ID>ERP162030</PRIMARY_ID>
      <SECONDARY_ID>PRJEB77669</SECONDARY_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION/>
    <SAMPLE_DESCRIPTOR accession="ERS25059179">
      <IDENTIFIERS>
        <PRIMARY_ID>ERS25059179</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioSample">SAMEA118572246</EXTERNAL_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME/>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <PAIRED/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <ILLUMINA>
      <INSTRUMENT_MODEL>Illumina NovaSeq X Plus</INSTRUMENT_MODEL>
    </ILLUMINA>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>ERS25059179</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>ERA33359780</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX14538612&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX14538612&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
  <EXPERIMENT_ATTRIBUTES>
    <EXPERIMENT_ATTRIBUTE>
      <TAG>ENA-STATUS-ID</TAG>
      <VALUE>4</VALUE>
    </EXPERIMENT_ATTRIBUTE>
  </EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>