Genome sc|0003916

Genomics

Accession

NCBI Assembly:GCA_044998965.1

Cultures

Strain sc|0040742

Type

Isolate Genome

Estimated Quality Metrics

• Completeness: 93.51%
• Contamination: 1.39%
• Quality: 86.56

Ribosomal and transfer RNA genes

• 2 16S rRNAs (up to 100.0%)
• 2 23S rRNAs (up to 100.0%)
• tRNAs for 20 amino acids

Sequencing depth

50.0 ×

Source

• BioSample: SAMN44467667

Other features

• G+C Content: 63.0%
• Coding Density: 86.17%
• Codon Table: 11
• N50: 6,633,211 bp
• Contigs: 2
• Largest Contig: 6,633,211 bp
• Assembly Length: 6,752,650 bp
• Ambiguous Assembly Fraction: 0.0%

Submitter comments

A total of 62,904 reads were generated through sequencing, with an N50 length of 9,919 bp. Adapter sequences from the Nanopore library were removed using Porechop (v0.2.4). Subsequent filtering with NanoFilt (v2.8.0) and further adapter trimming resulted in a refined dataset of 58,611 reads. These long reads were then assembled de novo using Flye assembler (v2.9.3-b1797), producing two contigs. The assembly was polished through four rounds of Racon (v1.5.0), followed by a final polishing step with Medaka (v2.0.1).

Automated checks

Complete

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Last modified 2 months ago

Sample Metadata

BioSample entries

• BioSample: SAMN44467667

Date

Collection Date: 2021-05-10

Location

Not detected

Toponym

Geo Loc Name: Morocco: Maamora forest)

Environment

Sample Type: Pure single cell culture

Isolation Source: root nodules of Ononis repens

Other

Host: Ononis spinosa subsp. procurrens

Package

Not detected

All retrieved samples

• SAMN44467667: Microbe sample from Mesorhizobium sp. ORM16

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Metadata retrieved 2 months ago