Genome quality was evaluated with CheckM v1.2.1 (Parks et al., 2015), which estimates completeness and contamination based on lineage-specific single-copy marker genes.
The G+C content was 64.41%. Ribosomal RNAs were detected with Barrnap v0.9, revealing two complete 16S, 23S, and 5S rRNA operons. Transfer RNAs were identified with tRNAscan-SE vX.X, covering all 20 standard amino acids.
Additional metrics were obtained during annotation: G+C content from the assembled sequence, rRNA genes with Barrnap, and tRNA coverage with tRNAscan-SE. These provide complementary information on gene recovery and coding capacity.
A potential limitation is that CheckM estimates can be biased if marker genes are missing from its database or present in multiple copies (e.g., on plasmids). rRNA operons may also be fragmented in assemblies, leading to incomplete recovery.