The information about genome coverage was received from the original authors via personal communication (see below). From: s-jkim@kordi.re.kr, Hyun-Myung Oh
Accoding to Dr. Kwon at kiost (
kkkwon@kiost.ac.kr) abi 3730 xl sequencing coverage was 2x from 4,512-clone genomic library and the additional pyrosequencing runs were done 3 times.
I could not get raw reads from pyrosequencing, I expect not more that 100k reads per 454 run.
Since Macrogen reported tens of thounsand reads per 454 run, and they did it three times to get more than 40 phred score, the following calculation would make sense !
100k * 3 run * 400 bp / 2.7 Mbp = 44.44 x
So our final decision on the genomic coverage of the completeted genome of IMCC1322 should be 46 folds.