Nanopore data has been generated from R10.4.1 chemistry flow cells. The reads have been basecalled in SUP mode

BioSample Accession(s)

SAMEA114263424, ERS16252363

ENA-CHECKLIST

ERC000011

organism

soil metagenome

collection date

2021-07-01

scientific_name

soil metagenome

geographic location (country and/or sea)

Denmark

HQ MAG for uncultured Acidobacteriota bacterium, recovered from an environmental sample from Denmark

BioSample Accession(s)

SAMEA114825218, ERS17250085

ENA-CHECKLIST

ERC000011

binning software

MetaBAT2, GraphMB, SemiBin2, DAS Tool, mmlong2

organism

uncultured Acidobacteriota bacterium

broad-scale environmental context

Fields

assembly quality

Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S and 5S rRNA genes and at least 18 tRNAs

collection date

2021-07-01

sample derived from

ERS16252363

geographic location (longitude)

9.6177

taxonomic identity marker

multi-marker approach

binning parameters

Ensemble iterative binning using coverage and kmer

completeness score

96.46

contamination score

3.05

environmental medium

not collected

geographic location (latitude)

56.7771

scientific_name

uncultured Acidobacteriota bacterium

local environmental context

Poales, grass

metagenomic source

soil metagenome

project name

Microflora Danica

completeness software

CheckM2

geographic location (country and/or sea)

Denmark

isolation_source

Soil

ENA-LAST-UPDATE

2023-12-07

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="ERX11420853" alias="ena-EXPERIMENT-TAB-12-09-2023-07:50:33:632-28873" center_name="Center for Microbial Communities">
  <IDENTIFIERS>
    <PRIMARY_ID>ERX11420853</PRIMARY_ID>
    <SUBMITTER_ID namespace="Center for Microbial Communities">ena-EXPERIMENT-TAB-12-09-2023-07:50:33:632-28873</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>PromethION sequencing</TITLE>
  <STUDY_REF accession="ERP143702">
    <IDENTIFIERS>
      <PRIMARY_ID>ERP143702</PRIMARY_ID>
      <SECONDARY_ID>PRJEB58634</SECONDARY_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION/>
    <SAMPLE_DESCRIPTOR accession="ERS16252363">
      <IDENTIFIERS>
        <PRIMARY_ID>ERS16252363</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioSample">SAMEA114263424</EXTERNAL_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>LIB-CHJ-R1041-MFD05942</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <SINGLE/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <OXFORD_NANOPORE>
      <INSTRUMENT_MODEL>PromethION</INSTRUMENT_MODEL>
    </OXFORD_NANOPORE>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>ERS16252363</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>ERA27205282</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX11420853&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
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      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
  <EXPERIMENT_ATTRIBUTES>
    <EXPERIMENT_ATTRIBUTE>
      <TAG>ENA-STATUS-ID</TAG>
      <VALUE>4</VALUE>
    </EXPERIMENT_ATTRIBUTE>
  </EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="ERX11327803" alias="ena-EXPERIMENT-TAB-30-08-2023-15:25:49:812-17435" center_name="Center for Microbial Communities">
  <IDENTIFIERS>
    <PRIMARY_ID>ERX11327803</PRIMARY_ID>
    <SUBMITTER_ID namespace="Center for Microbial Communities">ena-EXPERIMENT-TAB-30-08-2023-15:25:49:812-17435</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>PromethION sequencing</TITLE>
  <STUDY_REF accession="ERP143702">
    <IDENTIFIERS>
      <PRIMARY_ID>ERP143702</PRIMARY_ID>
      <SECONDARY_ID>PRJEB58634</SECONDARY_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION/>
    <SAMPLE_DESCRIPTOR accession="ERS16252363">
      <IDENTIFIERS>
        <PRIMARY_ID>ERS16252363</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioSample">SAMEA114263424</EXTERNAL_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>LIB-CHJ-R1041-MFD05942</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <SINGLE/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <OXFORD_NANOPORE>
      <INSTRUMENT_MODEL>PromethION</INSTRUMENT_MODEL>
    </OXFORD_NANOPORE>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>ERS16252363</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>ERA27127941</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX11327803&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX11327803&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
  <EXPERIMENT_ATTRIBUTES>
    <EXPERIMENT_ATTRIBUTE>
      <TAG>ENA-STATUS-ID</TAG>
      <VALUE>4</VALUE>
    </EXPERIMENT_ATTRIBUTE>
  </EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="ERX11327804" alias="ena-EXPERIMENT-TAB-30-08-2023-15:25:49:813-17437" center_name="Center for Microbial Communities">
  <IDENTIFIERS>
    <PRIMARY_ID>ERX11327804</PRIMARY_ID>
    <SUBMITTER_ID namespace="Center for Microbial Communities">ena-EXPERIMENT-TAB-30-08-2023-15:25:49:813-17437</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>PromethION sequencing</TITLE>
  <STUDY_REF accession="ERP143702">
    <IDENTIFIERS>
      <PRIMARY_ID>ERP143702</PRIMARY_ID>
      <SECONDARY_ID>PRJEB58634</SECONDARY_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION/>
    <SAMPLE_DESCRIPTOR accession="ERS16252363">
      <IDENTIFIERS>
        <PRIMARY_ID>ERS16252363</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioSample">SAMEA114263424</EXTERNAL_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>LIB-CHJ-R1041-MFD05942</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <SINGLE/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <OXFORD_NANOPORE>
      <INSTRUMENT_MODEL>PromethION</INSTRUMENT_MODEL>
    </OXFORD_NANOPORE>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>ERS16252363</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>ERA27127941</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX11327804&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX11327804&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
  <EXPERIMENT_ATTRIBUTES>
    <EXPERIMENT_ATTRIBUTE>
      <TAG>ENA-STATUS-ID</TAG>
      <VALUE>4</VALUE>
    </EXPERIMENT_ATTRIBUTE>
  </EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="ERX11327805" alias="ena-EXPERIMENT-TAB-30-08-2023-15:25:49:813-17439" center_name="Center for Microbial Communities">
  <IDENTIFIERS>
    <PRIMARY_ID>ERX11327805</PRIMARY_ID>
    <SUBMITTER_ID namespace="Center for Microbial Communities">ena-EXPERIMENT-TAB-30-08-2023-15:25:49:813-17439</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>PromethION sequencing</TITLE>
  <STUDY_REF accession="ERP143702">
    <IDENTIFIERS>
      <PRIMARY_ID>ERP143702</PRIMARY_ID>
      <SECONDARY_ID>PRJEB58634</SECONDARY_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION/>
    <SAMPLE_DESCRIPTOR accession="ERS16252363">
      <IDENTIFIERS>
        <PRIMARY_ID>ERS16252363</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioSample">SAMEA114263424</EXTERNAL_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>LIB-CHJ-R1041-MFD05942</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <SINGLE/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <OXFORD_NANOPORE>
      <INSTRUMENT_MODEL>PromethION</INSTRUMENT_MODEL>
    </OXFORD_NANOPORE>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>ERS16252363</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>ERA27127941</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX11327805&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX11327805&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
  <EXPERIMENT_ATTRIBUTES>
    <EXPERIMENT_ATTRIBUTE>
      <TAG>ENA-STATUS-ID</TAG>
      <VALUE>4</VALUE>
    </EXPERIMENT_ATTRIBUTE>
  </EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>