Keywords: GSC:MIxS;MIMAG:6.0

BioSample Accession(s)

SAMN52030977

collection_date

2021

env_broad_scale

bioreactor [ENVO:00002123]

env_local_scale

anaerobic bioreactor [ENVO:00002124]

env_medium

anaerobic digester sludge [ENVO:00003965]

environmental-sample

1

geo_loc_name

Luxembourg:Belvaux

isolate

Cloaci_S8

isolation_source

enrichment culture of bioreactor seeded with anaerobic digester sludge

lat_lon

49.506945 N 5.943900 E

metagenome-source

anaerobic digester metagenome

metagenomic

1

sample-type

metagenomic assembly

modified medium DSM61 supplemented with antibiotics, culture at RT, soft shaking, initial sample collected 2021-10-06, culture started 2021-11-09

BioSample Accession(s)

SRS26726282, SAMN51204464

collection_date

2021-09-12

geo_loc_name

Luxembourg:Belvaux

isolation_source

enrichment culture derived from bioreactor seeded with anaerobic digester sludge

lat_lon

49.506945 N 5.943900 E

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="SRX30708453" alias="cloaci_enrichment_wgs" center_name="SUB15609802" broker_name="NCBI">
  <IDENTIFIERS>
    <PRIMARY_ID>SRX30708453</PRIMARY_ID>
    <SUBMITTER_ID namespace="SUB15609802">cloaci_enrichment_wgs</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>Metagenomic sequencing of enrichment culture</TITLE>
  <STUDY_REF accession="SRP617795">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP617795</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject">PRJNA1320513</EXTERNAL_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION>miseq 300 bps, reagent kit v3</DESIGN_DESCRIPTION>
    <SAMPLE_DESCRIPTOR accession="SRS26726282">
      <IDENTIFIERS>
        <PRIMARY_ID>SRS26726282</PRIMARY_ID>
        <SUBMITTER_ID namespace="pda|malteherold@orcid">cloaci_enrichment</SUBMITTER_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>cloaci_enrichment_wgs</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>GENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <PAIRED/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <ILLUMINA>
      <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
    </ILLUMINA>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>SRS26726282</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>SRA2239138</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX30708453&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX30708453&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
  <EXPERIMENT_ATTRIBUTES>
    <EXPERIMENT_ATTRIBUTE>
      <TAG>ENA-STATUS</TAG>
      <VALUE>PUBLIC</VALUE>
    </EXPERIMENT_ATTRIBUTE>
  </EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>