The genome was assembled using SPAdes and Canu , using default settings for both MinION and Illumina data. For the MinION data, Nanopolish was used to improve the consensus sequence. The assembly resulted in three large contigs which were aligned against the closest
reference genomes using Mauve. This allowed a prediction of the orientation of the three contigs, and gaps were subsequently closed by long-range PCR using the MasterAmp extra-long PCR kit and Sanger sequencing.