The genome size is 2.97 Mbp, G+C percentage is 56.43%, with 100.0% completeness and 0.0% contamination. The isolate was determined to be related to Faecalibacterium prausnitzii (98.08%) based on 16S rRNA gene analysis. ANI comparison to F. prausnitzii was just below species level assignment (94.46%), and GTDB-Tk classification as ‘Faecalibacterium prausnitzii_J’ recommended the creation of a novel species. Functional analysis showed the strain has 135 transporters, 18 secretion genes, and predicted utilization of starch and production of L-glutamate, riboflavin, and cobalamin. Production of butyrate (4.74 ± 0.30 mM) was confirmed for strain CLA-AA-H281 when grown in YCFA broth (DSMZ Medium No. 1611) in Hungate tubes for 48 hours at 37 °C. In total, 159 CAZymes were identified, with 27 different glycoside hydrolase families and 12 glycoside transferase families represented. The strain CLA-AA-H281 (phylum Bacillota, family Oscillospiraceae) was isolated from human faeces.