Type strain is Rhodoluna miladensis MiE-23b (GCA_965234705.1), isolated from 5 m depth from Lake Milada, Czechia (date: 2019-10-15), via high-throughput dilution to extinction cultivation. MiE-23b has a genome size of 1.28 Mbp with a genomic GC content of 50.2%, contains 3 rRNA genes and 40 tRNAs. The genome is complete, consisting of a circular chromosome. The genome contains genes encoding rhodopsins but lacks the biosynthetic pathway for retinal biosynthesis. No genes for flagella or pilus assembly and chemotaxis were annotated. Pathways for the biosynthesis of all amino acids except for methionine and histidine were predicted. Further, pathways for riboflavin, pyridoxal, coenzyme A, and menaquinone were identified. The closest cultivated relative is Rhodoluna lacicola MWH-Ta8 (GCF_000699505), with an average amino acid identity of 65.06% and average nucleotide identity of 67.72%. Current GTDB classification (R220): d__Bacteria; p__Actinomycetota; c__Actinomycetia; o__Actinomycetales; f__Microbacteriaceae; g__Rhodoluna; s__.