A novel halotolerant actinomycete, designated strain BNT52T, was isolated from soil collected from Cihanbeyli Salt Mine in the central Anatolia region of Turkey, and examined using a polyphasic taxonomic approach. The isolate was found to have chemical and morphological properties typical of the genus
Amycolatopsis
and formed a distinct phyletic line in the 16S rRNA gene tree. Strain BNT52T was most closely related to
Amycolatopsis nigrescens
CSC17Ta-90T (96.7 %),
Amycolatopsis magusensis
KT2025T (96.6 %),
Amycolatopsis sulphurea
DSM 46092T (96.6 %),
Amycolatopsis dongchuanensis
YIM 75904T (96.5 %),
Amycolatopsis ultiminotia
RP-AC36T (96.4 %) and
Amycolatopsis sacchari
DSM 44468T (96.4 %). Sequence similarities with other strains of species of the genus
Amycolatopsis
were lower than 96.2 %. The isolate grew at 20–37 °C, pH 6–12 and in the presence of 0–10 % (w/v) NaCl. The cell wall of the novel strain contained meso-diaminopimelic acid and arabinose and galactose as the diagnostic sugars. Major fatty acids were iso-C16 : 0 2-OH and iso-C16 : 0. The predominant menaquinone was MK-9(H4). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylmethylethanolamine. The genomic DNA G+C content was 68.8 mol%. On the basis of the data from this polyphasic taxonomic study, strain BNT52T represents a novel species within the genus
Amycolatopsis
for which the name
Amycolatopsis
cihanbeyliensis sp. nov. is proposed (type strain BNT52T = KCTC 29065T = NRRL B-24886T = DSM 45679T).