Tramway Ridge Site A, 2-4 cm depth

BioSample Accession(s)

ERS223619

ENA-CHECKLIST

ERC000021

amount or size of sample collected

0.05

environment (material)

soil

organism

metagenome

collection date

2009-02

target gene

16S rRNA

geographic location (longitude)

167.111367

sediment environmental package

sediment

geographic location (latitude)

-77.518383

scientific_name

metagenome

geographic location (country and/or sea,region)

Antarctica

geographic location (elevation)

3370

investigation type

metagenome

ENA-SUBMISSION-TOOL

SRA-Webin

depth

0.03

ENA-FIRST-PUBLIC

2014-05-12

project name

Novel subsurface lineages dominate fumarolic microbial communities at Tramway Ridge, Mt. Erebus, Antarctica

sequencing method

Pyrosequencing

ENA-LAST-UPDATE

2018-03-09

pH

8.52

temperature

65

nucleic acid extraction

CTAB-BME (Barrett, 2006)

environment (biome)

terrestrial geothermal fumarole

environment (feature)

fumarole

Metadata as EBI XML

<RUN_SET>
<RUN accession="SRR6519254" alias="ATGGCTT.2.fastq.gz" center_name="SUB3589185" broker_name="NCBI">
  <IDENTIFIERS>
    <PRIMARY_ID>SRR6519254</PRIMARY_ID>
    <SUBMITTER_ID label="0" namespace="SUB3589185">ATGGCTT.2.fastq.gz</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>Illumina HiSeq 1000 paired end sequencing; Metagenome of Mt. Erebus fumaroloes: site A subsurface</TITLE>
  <EXPERIMENT_REF accession="SRX3606830">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX3606830</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB3589185">A_sub_Ill_1</SUBMITTER_ID>
    </IDENTIFIERS>
  </EXPERIMENT_REF>
  <RUN_LINKS>
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      <XREF_LINK>
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        <ID>SRA653124</ID>
      </XREF_LINK>
    </RUN_LINK>
    <RUN_LINK>
      <XREF_LINK>
        <DB>ENA-STUDY</DB>
        <ID>SRP131651</ID>
      </XREF_LINK>
    </RUN_LINK>
    <RUN_LINK>
      <XREF_LINK>
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        <ID>ERS223619</ID>
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    </RUN_LINK>
    <RUN_LINK>
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      </XREF_LINK>
    </RUN_LINK>
  </RUN_LINKS>
  <RUN_ATTRIBUTES>
    <RUN_ATTRIBUTE>
      <TAG>ENA-FIRST-PUBLIC</TAG>
      <VALUE>2019-07-01</VALUE>
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    <RUN_ATTRIBUTE>
      <TAG>ENA-LAST-UPDATE</TAG>
      <VALUE>2019-07-01</VALUE>
    </RUN_ATTRIBUTE>
  </RUN_ATTRIBUTES>
</RUN>
</RUN_SET>

Metadata as EBI XML

<RUN_SET>
<RUN accession="SRR6519255" alias="TGCAACT.2.fastq.gz" center_name="SUB3589185" broker_name="NCBI">
  <IDENTIFIERS>
    <PRIMARY_ID>SRR6519255</PRIMARY_ID>
    <SUBMITTER_ID label="3" namespace="SUB3589185">TGCAACT.2.fastq.gz</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>Illumina HiSeq 1000 paired end sequencing; Metagenome of Mt. Erebus fumaroloes: site A subsurface</TITLE>
  <EXPERIMENT_REF accession="SRX3606829">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX3606829</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB3589185">A_sub_Ill_2</SUBMITTER_ID>
    </IDENTIFIERS>
  </EXPERIMENT_REF>
  <RUN_LINKS>
    <RUN_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>SRA653124</ID>
      </XREF_LINK>
    </RUN_LINK>
    <RUN_LINK>
      <XREF_LINK>
        <DB>ENA-STUDY</DB>
        <ID>SRP131651</ID>
      </XREF_LINK>
    </RUN_LINK>
    <RUN_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>ERS223619</ID>
      </XREF_LINK>
    </RUN_LINK>
    <RUN_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
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      </XREF_LINK>
    </RUN_LINK>
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      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR6519255&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </RUN_LINK>
  </RUN_LINKS>
  <RUN_ATTRIBUTES>
    <RUN_ATTRIBUTE>
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      <VALUE>2019-07-01</VALUE>
    </RUN_ATTRIBUTE>
    <RUN_ATTRIBUTE>
      <TAG>ENA-LAST-UPDATE</TAG>
      <VALUE>2019-07-01</VALUE>
    </RUN_ATTRIBUTE>
  </RUN_ATTRIBUTES>
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</RUN_SET>