Metadata as EBI XML
<EXPERIMENT_SET>
<EXPERIMENT accession="SRX1847093" alias="butane50_ATGTCA" center_name="Ribocon GmbH" broker_name="NCBI">
<IDENTIFIERS>
<PRIMARY_ID>SRX1847093</PRIMARY_ID>
<SUBMITTER_ID namespace="Ribocon GmbH">butane50_ATGTCA</SUBMITTER_ID>
</IDENTIFIERS>
<TITLE>Paired end sequences</TITLE>
<STUDY_REF accession="SRP076597">
<IDENTIFIERS>
<PRIMARY_ID>SRP076597</PRIMARY_ID>
<EXTERNAL_ID namespace="BioProject">PRJNA319143,PRJNA319143</EXTERNAL_ID>
</IDENTIFIERS>
</STUDY_REF>
<DESIGN>
<DESIGN_DESCRIPTION>Genomic DNA was extracted from 15 ml of the Butane50 culture using the FastDNA Spin Kit for Soil (MP Biomedicals, Illkirch, France). For paired-end library preparation the TruSeq DNA PCR-Free Sample Prep Kit (Illumina) was used including the following modifications of the manufacturer’s guidelines: A total amount of 700 ng DNA (in 50 µl volume) was fragmented in 500 µl nebulization buffer (50% glycerol v/v, 35 mM Tris-HCl, 5 mM EDTA), using a Nebulizer (Roche), with a fragmentation time of 3 min, and applied pressure of 32 psi. The fragmented DNA was purified via a MinElute purification column (Qiagen). Following end repair, the first size selection step (removal of large DNA fragments) was done with a sample purification bead/H2O mixture of 6/5 (v/v).</DESIGN_DESCRIPTION>
<SAMPLE_DESCRIPTOR accession="SRS1505411">
<IDENTIFIERS>
<PRIMARY_ID>SRS1505411</PRIMARY_ID>
<EXTERNAL_ID namespace="BioSample">SAMN05004607</EXTERNAL_ID>
</IDENTIFIERS>
</SAMPLE_DESCRIPTOR>
<LIBRARY_DESCRIPTOR>
<LIBRARY_NAME/>
<LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
<LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
<LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
<LIBRARY_LAYOUT>
<PAIRED NOMINAL_SDEV="0.0E0"/>
</LIBRARY_LAYOUT>
</LIBRARY_DESCRIPTOR>
<SPOT_DESCRIPTOR>
<SPOT_DECODE_SPEC>
<SPOT_LENGTH>600</SPOT_LENGTH>
<READ_SPEC>
<READ_INDEX>0</READ_INDEX>
<READ_CLASS>Application Read</READ_CLASS>
<READ_TYPE>Forward</READ_TYPE>
<BASE_COORD>1</BASE_COORD>
</READ_SPEC>
<READ_SPEC>
<READ_INDEX>1</READ_INDEX>
<READ_CLASS>Application Read</READ_CLASS>
<READ_TYPE>Reverse</READ_TYPE>
<BASE_COORD>301</BASE_COORD>
</READ_SPEC>
</SPOT_DECODE_SPEC>
</SPOT_DESCRIPTOR>
</DESIGN>
<PLATFORM>
<ILLUMINA>
<INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
</ILLUMINA>
</PLATFORM>
<EXPERIMENT_LINKS>
<EXPERIMENT_LINK>
<XREF_LINK>
<DB>ENA-SAMPLE</DB>
<ID>SRS1505411</ID>
</XREF_LINK>
</EXPERIMENT_LINK>
<EXPERIMENT_LINK>
<XREF_LINK>
<DB>ENA-SUBMISSION</DB>
<ID>SRA433584</ID>
</XREF_LINK>
</EXPERIMENT_LINK>
<EXPERIMENT_LINK>
<XREF_LINK>
<DB>ENA-FASTQ-FILES</DB>
<ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX1847093&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
</XREF_LINK>
</EXPERIMENT_LINK>
<EXPERIMENT_LINK>
<XREF_LINK>
<DB>ENA-SUBMITTED-FILES</DB>
<ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX1847093&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
</XREF_LINK>
</EXPERIMENT_LINK>
</EXPERIMENT_LINKS>
<EXPERIMENT_ATTRIBUTES>
<EXPERIMENT_ATTRIBUTE>
<TAG>ENA-STATUS-ID</TAG>
<VALUE>4</VALUE>
</EXPERIMENT_ATTRIBUTE>
</EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>
Metadata retrieved about 2 months ago,
sequencing experiment 2100