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Authors Fernández

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Fernández, Franco Daniel


Publications
7

CitationNamesAbstract
Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization Fernández et al. (2024). Microorganisms 12 (1) Ca. Phytoplasma meliae
Genome characterization of ‘Candidatus Phytoplasma meliae’ (isolate ChTYXIII) Fernández, Conci (2021). Ca. Phytoplasma meliae
Draft Genome Sequence of “ Candidatus Phytoplasma pruni” (X-Disease Group, Subgroup 16SrIII-B) Strain ChTDIII from Argentina Fernández et al. (2020). Microbiology Resource Announcements 9 (38) Ca. Phytoplasma pruni
“First report of ‘Candidatus Phytoplasma pyri’-related strain causing pear decline in Argentina" Fernández, Conci (2019). Crop Protection 121 Ca. Phytoplasma pyri
‘Candidatus Phytoplasma platensis’, a novel taxon associated with daisy (Bellis perennis) virescence and related diseases in South America Fernandez et al. (2019). Ca. Phytoplasma Ca. Phytoplasma platensis Ca. Phytoplasma pruni
First report of a ‘Candidatus phytoplasma pyri’ strain in Argentina Fernández et al. (2017). Australasian Plant Disease Notes 12 (1)
Description of ‘Candidatus Phytoplasma meliae’, a phytoplasma associated with Chinaberry (Melia azedarach L.) yellowing in South America Fernández et al. (2016). International Journal of Systematic and Evolutionary Microbiology 66 (12) Ca. Phytoplasma meliae

Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization
‘Candidatus Phytoplasma meliae’ is a pathogen associated with chinaberry yellowing disease, which has become a major phytosanitary problem for chinaberry forestry production in Argentina. Despite its economic impact, no genome information of this phytoplasma has been published, which has hindered its characterization at the genomic level. In this study, we used a metagenomics approach to analyze the draft genome of the ‘Ca. P. meliae’ strain ChTYXIII. The draft assembly consisted of twenty-one contigs with a total length of 751.949 bp, and annotation revealed 669 CDSs, 34 tRNAs, and 1 set of rRNA operons. The metabolic pathways analysis showed that ChTYXIII contains the complete core genes for glycolysis and a functional Sec system for protein translocation. Our phylogenomic analysis based on 133 single-copy genes and genome-to-genome metrics supports the classification as unique ‘Ca. P. species’ within the MPV clade. We also identified 31 putative effectors, including a homolog to SAP11 and others that have only been described in this pathogen. Our ortholog analysis revealed 37 PMU core genes in the genome of ‘Ca. P. meliae’ ChTYXIII, leading to the identification of 2 intact PMUs. Our work provides important genomic information for ‘Ca. P. meliae’ and others phytoplasmas for the 16SrXIII (MPV) group.
Genome characterization of ‘Candidatus Phytoplasma meliae’ (isolate ChTYXIII)
Abstract‘Candidatus Phytoplasma meliae’ (subgroups 16SrXIII-G and XIII-C) has been reported in association to chinaberry yellowing disease in Argentina, Bolivia and Paraguay. In Argentina, this disease constitutes a major phytosanitary problem for chinaberry forestry production. To date, no genome information of this phytoplasma and others from 16SrXIII-group has been published, hindered its characterization at genomic level. Here we analyze the draft genome of ‘Candidatus Phytoplasma meliae’ strain ChTYXIII obtained from a chinaberry-infected plant using a metagenomics approach. The draft assembly consists of twenty-one contigs with a total length of 751.949 bp. The annotation contains 669 CDSs, 34tRNA and one set of rRNA operons. Metabolic pathways analysis indicated that the ChTYXIII contains the complete core genes for glycolysis and functional sec system for translocation of proteins. The phylogenetic relationships inferred 132 single copy genes (orthologues core) analysis revealed that ‘Ca. P. meliae’ constitutes a clade closely related to the ‘Ca. australiense’ and ‘Ca. P. solani’. Thirty-one putative effectors were identified, among which a homologue to SAP11 was found and others that have only been described in this pathogen. This work provides relevant genomic information for ‘Ca. P. meliae’ and constitutes the first genome described for the group 16SrXIII (MPV).
Draft Genome Sequence of “ Candidatus Phytoplasma pruni” (X-Disease Group, Subgroup 16SrIII-B) Strain ChTDIII from Argentina
Herein, we report the draft genome sequence of “ Candidatus Phytoplasma pruni” strain ChTDIII (subgroup 16SrIII-B). The final assembly consists of 790,517 nucleotides organized in 67 contigs (minimal size, 1 kb), with a G+C content of 29.4% and encoding 672 proteins.
‘Candidatus Phytoplasma platensis’, a novel taxon associated with daisy (Bellis perennis) virescence and related diseases in South America
AbstractBellis perennis virescence (BellVir) phytoplasma affects ornamental daisies in Argentina. It has been previously classified within the X-disease group, subgroup III-J, which is one of the most important and widely distributed in South America, affecting diverse plant hosts. In this study, we compared 16S rRNA, ribosomal proteins rpIV and rps3, secA and immunodominant proteins imp and idpA genes of BellVir phytoplasma with previously described ‘Candidatus Phytoplasma’ species. The 16S rRNA gene of strain BellVir shared less than 97.5% with all previously described ‘Ca. Phytoplasma’ taxa except for ‘Ca. Phytoplasma pruni’. According to the recommended rules for the description of novel taxa within ‘Ca. Phytoplasma’, it should be considered as ‘Ca. P. pruni’-related strain. However, multilocus analysis showed further molecular diversity that distinguished BellVir phytoplasma from ‘Ca. Phytoplasma pruni’. Besides, BellVir phytoplasma and 16SrIII-J related strains have a geographical distribution restricted to South America, where ‘Ca. P.pruni’ has not been detected. Two insect vectors have been reported to transmit 16SrIII-J phytoplasmas, which have not been found to transmit ‘Ca. Phytoplasma pruni’. Having a wide host range, they have not been detected in Prunus persica. Therefore, based on multilocus sequence analyses, specific vector transmission and geographical distribution, we propose the recognition of the novel phytoplasma species ‘Ca. Phytoplasma platensis’, within the X-disease clade, with Bellis perennis virescence phytoplasma as the reference strain.
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