Zheng, Desen


Publications
2

Comparative RT-qPCR and qPCR reveals early infection, low-titer infection, and relative cell activity of the HLB bacterium,CandidatusLiberibacter asiaticus

Citation
Patterson et al. (2024).
Names
Ca. Liberibacter asiaticus
Abstract
AbstractCandidatusLiberibacter asiaticus (Las) is one of the causal agents of citrus huanglongbing (HLB) epidemics worldwide. Due to its fastidious nature, intracellular and systemic infection, detecting Las at early and/or low-titer infection, as well as differentiating between live or dead cells in the host psyllids and citrus plants is critical for effective HLB management. To achieve both sensitive Las detection and differentiation, we employed one-step reverse transcription-quantitative PCR

Towards the completion of Koch's postulates for the citrus huanglongbing bacterium, Candidatus Liberibacter asiaticus

Citation
Zheng et al. (2024). Horticulture Research 11 (3)
Names
Ca. Liberibacter asiaticus
Abstract
Abstract Candidatus Liberibacter asiaticus (Las) is one of the causal agents of huanglongbing (HLB), the most devastating disease of citrus worldwide. Due to the intracellular lifestyle and significant genome reduction, culturing Las in vitro has proven to be extremely challenging. In this study, we optimized growth conditions and developed a semi-selective medium based on the results of nutritional and antibiotic screening assays. Using these optimized conditions, we were able to