Keywords: GSC:MIxS;MIMS:6.0

BioSample Accession(s)

SRS19719933

collection_date

2011

INSDC secondary accession

SRS19719933

NCBI submission package

MIMS.me.sediment.6.0

depth

0

organism

hot springs metagenome

elev

missing

env_broad_scale

hot spring [ENVO:00000051]

geo_loc_name

China: Gumingquan spring, Tengchong, Yunnan

env_medium

microbial feature [ENVO_01000007]

lat_lon

24.57060 N 98.43615 E

env_local_scale

microbial feature [ENVO_01000007]

isolation_source

microbial streamers

BioSampleModel

MIGS/MIMS/MIMARKS.sediment

ENA-FIRST-PUBLIC

2023-12-04

ENA-LAST-UPDATE

2023-12-06

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="SRX22731270" alias="GMQ_2011" center_name="SUB14015752" broker_name="NCBI">
  <IDENTIFIERS>
    <PRIMARY_ID>SRX22731270</PRIMARY_ID>
    <SUBMITTER_ID namespace="SUB14015752">GMQ_2011</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>Gumingquan 2011 streamer sample sequence reads</TITLE>
  <STUDY_REF accession="SRP475647">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP475647</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB14015752">bp0</SUBMITTER_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION>DNA was extracted from 0.5 to 1 g (wet weight) of sediment and biomass-containing filters using a FastDNA spin kit for soil (MP Biomedical, OH, USA)according to the manufacturers instructions. DNA quality was assessed using a NanoDrop ND-1000spectrophotometer (NanoDrop Technology, USA). The extracted DNA was sheared to 500 bp using aCovaris Ultrasonicator (Covaris Inc., Woburn, MA, USA) following the manufacturers recommendation. The sheared DNA was then subjected to end pairing and to adaptor ligation with multiplexing and was purified using an Ovation SP ultralow DR multiplex system.</DESIGN_DESCRIPTION>
    <SAMPLE_DESCRIPTOR accession="SRS19719933">
      <IDENTIFIERS>
        <PRIMARY_ID>SRS19719933</PRIMARY_ID>
        <SUBMITTER_ID namespace="pda|marikepalmer">GMQ 2011</SUBMITTER_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>GMQ_2011</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <PAIRED/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <ILLUMINA>
      <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
    </ILLUMINA>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>SRS19719933</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>SRA1762106</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX22731270&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX22731270&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
</EXPERIMENT>
</EXPERIMENT_SET>