In situ ammonia-fiber explosion-treated corn stover incubated for 6 months in Great Boiling Spring, NV, USA

collection_date

2016-03-29

temp

86.5

INSDC secondary accession

SRS11062446

organism

hot springs metagenome

isolation-source

enrichment culture; in situ ammonia-fiber explosion-treated corn stover incubated for 6 months in hot spring

env_broad_scale

biome [ENVO:00000428]|aquatic biome [ENVO:00002030]

env_local_scale

hot spring [ENVO:00000051]

NCBI submission package

MIMS.me.water.5.0

depth

0

ph

7.15

geo_loc_name

USA: Great Boiling Spring, Gerlach, Nevada

env_medium

enrichment culturing unit [ENVO:01001818]

lat_lon

40.662186 N 119.366729 W

BioSampleModel

MIGS/MIMS/MIMARKS.water

ENA-FIRST-PUBLIC

2021-11-12

ENA-LAST-UPDATE

2021-11-18

Metadata as EBI XML

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  <TITLE>Long-read metagenomic sequencing of Mar2016 GBS CS enrichment lab culture</TITLE>
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  <DESIGN>
    <DESIGN_DESCRIPTION>The library was prepared on DNA extracted from a subculture of the in situ corn stover enrichment following the Native Barcoding (EXP-NBD103) and Ligation Sequencing (SQK-LSK108) Kit 1D protocols according to manufacturers instructions, and sequencing was performed on a MinION FLO-MIN106 flow cell (Oxford Nanopore, Oxford Science Park, UK).</DESIGN_DESCRIPTION>
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Metadata as EBI XML

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