Wolframiiraptor gerlachensisTs


Citation

Formal styling
Wolframiiraptor gerlachensisTs Buessecker et al., 2022
Effective publication
Buessecker et al., 2022
SeqCode status
Valid (SeqCode)
Register List
seqco.de/r:slp2ijs4 (validated)
Canonical URL
https://seqco.de/i:22816

Nomenclature

Rank
Species
Syllabication
ger.lach.en'sis
Etymology
N.L. masc. adj. gerlachensis, of Gerlach, the region where Great Boiling Spring is located in Nevada, and this organism was obtained from
Nomenclatural type
NCBI Assembly: GCA_021323375.2
Nomenclatural status
Validly published under the SeqCode

Taxonomy

Description
A MAG representing this species was recovered from metagenomic sequencing of a stable enrichment culture, established from an in situ corn stover enrichment from Great Boiling Spring, Nevada, USA. Enrichment and maintenance of this species within the mixed-culture community was optimal at an incubation temperature of 80 °C with lignocellulose and sugars, at circumneutral pH. This species is dependent on tungsten for growth, with significant decline in its abundance within the community without tungsten added to the growth medium. Additionally, several tungstoenzymes conserved within the genus were expressed at a higher rate during growth on corn stover, suggesting direct involvement of tungstoenzymes in complex carbohydrate metabolism. Cells of this organism showed significant isotope enrichment when grown on isotopically labeled xylose-amended medium, with limited isotope enrichment during growth on medium amended with amino acids, glucose, ribose, and starch, indicating preferential assimilation of xylose. The genome sequence for this organism is 1,277,965 bp in size, and consists of 27 contigs and has a G+C content of 52 %. Completeness is estimated at 98.06 % with 0.49 % contamination, as estimated with CheckM. ANI comparisons among this genome and those of closely related species were below 86 %, supporting the delineation of this taxon as unique and distinct to other species in the genus. 
Classification
Incertae sedis (Archaea) » “Caldarchaeales” » Wolframiiraptoraceae » Wolframiiraptor » Wolframiiraptor gerlachensisTs
Parent
Wolframiiraptor gtdb

Genomics

Accession
NCBI Assembly:GCA_021323375.2
Type
Metagenome-Assembled Genome (MAG)
Estimated Quality Metrics
  • Completeness: 98.06%
  • Contamination: 0.49%
  • Quality: 95.61
Ribosomal and transfer RNA genes
  • 2 16S rRNAs (up to 100.0%)
  • 2 23S rRNAs (up to 51.0%)
  • tRNAs for 19 amino acids
Sequencing depth
12.0 ×
Source
Other features
  • G+C Content: 52.0%
  • Coding Density: 88.92%
  • Codon Table: 11
  • N50: 86,582 bp
  • Contigs: 27
  • Largest Contig: 149,570 bp
  • Assembly Length: 1,277,965 bp
  • Ambiguous Assembly Fraction: 0.0%
Automated checks
Complete
See additional details

Last modified 5 days ago

Metadata

Outside links and data sources
Search sequences
Local history
Registered by
Palmer, Marike about 3 years ago
Submitted by
Palmer, Marike about 2 years ago
Curators
Validated by
Rodriguez-R, Luis M about 2 years ago
Date of priority
2022-09-20 03:37 PM (UTC)

Publications
1

Citation Title
Buessecker et al., 2022, Nature Communications An essential role for tungsten in the ecology and evolution of a previously uncultivated lineage of anaerobic, thermophilic Archaea
Effective publication



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