Keywords: GSC:MIxS;MIMAG:6.0

BioSample Accession(s)

SAMN30287279, ERS16752032

collection_date

2014-06

number_of_host_metagenomes

3

organism

Arenicellales bacterium

isolation-source

sponge tissue, time zero, i.e. control

env_broad_scale

coral reef [ENVO:00000150]

metagenomic

1

derived-from

SAMN31806789, SAMN31806790, SAMN31806791

environmental-sample

1

env_local_scale

coral reef [ENVO:00000150]

metagenome-source

sponge metagenome

isolate

r_caze12_13

NCBI submission package

MIMAG.host-associated.6.0

experimental_conditions

time zero, i.e. control

host

Phyllospongia foliascens

geo_loc_name

Australia: Davies Reef, Great Barrier Reef

env_medium

sponge

lat_lon

18.816 S 147.633 E

sample-type

metagenomic assembly

BioSampleModel

MIMAG.host-associated

ENA-FIRST-PUBLIC

2022-08-12

ENA-LAST-UPDATE

2023-09-03

BioSample Accession(s)

SAMN31806789, SRS15847088

collection_date

2014-06

replicate

1

INSDC secondary accession

SRS15847088

NCBI submission package

Metagenome.environmental.1.0

organism

sponge metagenome

isolation-source

sponge tissue time zero, sample 1

host

Phyllospongia foliascens

geo_loc_name

Australia: Davies Reef, Great Barrier Reef

lat_lon

18.816 S 147.633 E

BioSampleModel

Metagenome or environmental

ENA-FIRST-PUBLIC

2022-11-23

ENA-LAST-UPDATE

2023-09-03

BioSample Accession(s)

SAMN31806790, SRS15847089

collection_date

2014-06

replicate

2

INSDC secondary accession

SRS15847089

NCBI submission package

Metagenome.environmental.1.0

organism

sponge metagenome

isolation-source

sponge tissue time zero, sample 2

host

Phyllospongia foliascens

geo_loc_name

Australia: Davies Reef, Great Barrier Reef

lat_lon

18.816 S 147.633 E

BioSampleModel

Metagenome or environmental

ENA-FIRST-PUBLIC

2022-11-23

ENA-LAST-UPDATE

2023-09-03

BioSample Accession(s)

SAMN31806791, SRS15847090

collection_date

2014-06

replicate

3

INSDC secondary accession

SRS15847090

NCBI submission package

Metagenome.environmental.1.0

organism

sponge metagenome

isolation-source

sponge tissue time zero, sample 3

host

Phyllospongia foliascens

geo_loc_name

Australia: Davies Reef, Great Barrier Reef

lat_lon

18.816 S 147.633 E

BioSampleModel

Metagenome or environmental

ENA-FIRST-PUBLIC

2022-11-23

ENA-LAST-UPDATE

2023-09-03

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="SRX18360459" alias="caze_1" center_name="SUB12300833" broker_name="NCBI">
  <IDENTIFIERS>
    <PRIMARY_ID>SRX18360459</PRIMARY_ID>
    <SUBMITTER_ID namespace="SUB12300833">caze_1</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>Carteriospongia foliascens time zero metagenome, sample 1</TITLE>
  <STUDY_REF accession="SRP228733">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP228733</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject">PRJNA588103</EXTERNAL_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION>Raw reads trimmed with Trimmomatic v0.33 with SLIDINGWINDOW:4:22 MINLEN:50 options</DESIGN_DESCRIPTION>
    <SAMPLE_DESCRIPTOR accession="SRS15847088">
      <IDENTIFIERS>
        <PRIMARY_ID>SRS15847088</PRIMARY_ID>
        <SUBMITTER_ID namespace="pda|nguyenviethung@orcid">caze_1</SUBMITTER_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>caze_1</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>unspecified</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <PAIRED/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <ILLUMINA>
      <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
    </ILLUMINA>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>SRS15847088</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>SRA1545887</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX18360459&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX18360459&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
</EXPERIMENT>
</EXPERIMENT_SET>

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="SRX18360460" alias="caze_2" center_name="SUB12300833" broker_name="NCBI">
  <IDENTIFIERS>
    <PRIMARY_ID>SRX18360460</PRIMARY_ID>
    <SUBMITTER_ID namespace="SUB12300833">caze_2</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>Carteriospongia foliascens time zero metagenome, sample 2</TITLE>
  <STUDY_REF accession="SRP228733">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP228733</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject">PRJNA588103</EXTERNAL_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION>Raw reads trimmed with Trimmomatic v0.33 with SLIDINGWINDOW:4:22 MINLEN:50 options</DESIGN_DESCRIPTION>
    <SAMPLE_DESCRIPTOR accession="SRS15847089">
      <IDENTIFIERS>
        <PRIMARY_ID>SRS15847089</PRIMARY_ID>
        <SUBMITTER_ID namespace="pda|nguyenviethung@orcid">caze_2</SUBMITTER_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>caze_2</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>unspecified</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <PAIRED/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <ILLUMINA>
      <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
    </ILLUMINA>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>SRS15847089</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>SRA1545887</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX18360460&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX18360460&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
</EXPERIMENT>
</EXPERIMENT_SET>

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="SRX18360461" alias="caze_3" center_name="SUB12300833" broker_name="NCBI">
  <IDENTIFIERS>
    <PRIMARY_ID>SRX18360461</PRIMARY_ID>
    <SUBMITTER_ID namespace="SUB12300833">caze_3</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>Carteriospongia foliascens time zero metagenome, sample 3</TITLE>
  <STUDY_REF accession="SRP228733">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP228733</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject">PRJNA588103</EXTERNAL_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION>Raw reads trimmed with Trimmomatic v0.33 with SLIDINGWINDOW:4:22 MINLEN:50 options</DESIGN_DESCRIPTION>
    <SAMPLE_DESCRIPTOR accession="SRS15847090">
      <IDENTIFIERS>
        <PRIMARY_ID>SRS15847090</PRIMARY_ID>
        <SUBMITTER_ID namespace="pda|nguyenviethung@orcid">caze_3</SUBMITTER_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>caze_3</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>unspecified</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <PAIRED/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <ILLUMINA>
      <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
    </ILLUMINA>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>SRS15847090</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>SRA1545887</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX18360461&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRX18360461&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
</EXPERIMENT>
</EXPERIMENT_SET>