Phytopathology®


Publications
80

Generous Hosts: ‘Candidatus Liberibacter asiaticus’ Growth in Madagascar Periwinkle (Catharanthus roseus) Highlights Its Nutritional Needs

Citation
Killiny (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’, the putative causal agent of citrus greening, is not available in pure culture yet. In addition to trees of citrus and citrus relatives, ‘Ca. L. asiaticus’ can grow in Madagascar periwinkle (Catharanthus roseus). Using gas chromatography-mass spectrometry, we compared the phloem sap composition in sweet orange ‘Valencia’ (Citrus sinensis) and periwinkle plants after the infection with ‘Ca. L. asiaticus’. Interestingly, in contrast to our previous studies of

Candidatus Liberibacter: From Movement, Host Responses, to Symptom Development of Citrus Huanglongbing

Citation
Pandey et al. (2022). Phytopathology® 112 (1)
Names
Liberibacter
Abstract
Candidatus Liberibacter spp. are fastidious α-proteobacteria that cause multiple diseases on plant hosts of economic importance, including the most devastating citrus disease: Huanglongbing (HLB). HLB was reported in Asia a century ago but has since spread worldwide. Understanding the pathogenesis of Candidatus Liberibacter spp. remains challenging as they are yet to be cultured in artificial media and infect the phloem, a sophisticated environment that is difficult to manipulate. Despite those

Effect of Plant Age, Temperature, and Vector Load on ‘Candidatus Liberibacter solanacearum’ in Planta Titer and Shoot Proliferation Symptoms in Carrot

Citation
Keshet-Sitton et al. (2022). Phytopathology® 112 (1)
Names
“Liberibacter solanacearum” Ca. Phytoplasma
Abstract
A decade ago, shoot proliferation symptoms (i.e., witches’ broom) in carrots were believed to be the cause of ‘Candidatus Phytoplasma’ and Spiroplasma infection, yet in recent years this association appeared to have weakened, and a closer association was found with the yet-unculturable, psyllid-transmitted Gram-negative bacterium ‘Candidatus Liberibacter solanacearum’. In Israel, carrots are grown throughout the year, yet shoot proliferation symptoms tend to appear only in mature plants and mos

Prevalent Transmission of ‘Candidatus Liberibacter asiaticus’ over ‘Ca. Liberibacter americanus’ in a Long-Term Controlled Environment

Citation
Gasparoto et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter americanus Ca. Liberibacter asiaticus
Abstract
In Brazil, citrus huanglongbing (HLB) is associated with ‘Candidatus Liberibacter americanus’ (CLam) and ‘Ca. Liberibacter asiaticus’ (CLas). However, there are few studies about HLB epidemiology when both Liberibacter spp. and its insect vector, the Asian citrus psyllid (ACP, Diaphorina citri), are present. The objective of this work was to compare the transmission of HLB by ACP when both CLam and CLas are present as primary inoculum. Two experiments were performed under screenhouse conditions

Identification of a Chromosomal Deletion Mutation and the Dynamics of Two Major Populations of ‘Candidatus Liberibacter asiaticus’ in Its Hosts

Citation
Armstrong et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (Las) is the prominent species of Liberibacter associated with huanglongbing, a devastating disease of citrus worldwide. In this study, we report the identification of an ∼8.3-kb DNA region of the Las genome containing eight putative open reading frames flanked by two inverted repeats, which was not present in the Las str. psy62 genome. Comparisons with other genome sequences established this region as a unique genetic element associated with genome plasticit

Host Plant Adaptation Drives Changes in Diaphorina citri Proteome Regulation, Proteoform Expression, and Transmission of ‘Candidatus Liberibacter asiaticus’, the Citrus Greening Pathogen

Citation
Ramsey et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
The Asian citrus psyllid (Diaphorina citri) is a pest of citrus and the primary insect vector of the bacterial pathogen, ‘Candidatus Liberibacter asiaticus’ (CLas), which is associated with citrus greening disease. The citrus relative Murraya paniculata (orange jasmine) is a host plant of D. citri but is more resistant to CLas compared with all tested Citrus genotypes. The effect of host switching of D. citri between Citrus medica (citron) and M. paniculata plants on the acquisition and transmi

Growth of ‘Candidatus Liberibacter asiaticus’ in Commercial Grapefruit Juice-Based Media Formulations Reveals Common Cell Density-Dependent Transient Behaviors

Citation
Merfa e Silva et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
The phloem-restricted, insect-transmitted bacterium ‘Candidatus Liberibacter asiaticus’ (CLas) is associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. The inability to culture CLas impairs the understanding of its virulence mechanisms and the development of effective management strategies to control this incurable disease. Previously, our research group used commercial grapefruit juice (GJ) to prolong the viability of CLas in vitro. In the present study, GJ was

Intracellular Life Cycle of ‘Candidatus Liberibacter asiaticus’ Inside Psyllid Gut Cells

Citation
Chun-Yi et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (CLas), the devastating pathogen related to Huanglongbing (HLB), is a phloem-limited, fastidious, insect-borne bacterium. Rapid spread of HLB disease relies on CLas-efficient propagation in the vector, the Asian citrus psyllid Diaphorina citri, in a circulative manner. Understanding the intracellular lifecycle of CLas in psyllid midgut, the major organ for CLas transmission, is fundamental to improving current management strategies. Using a microscopic approa

The Impact of Diaphorina citri-Vectored ‘Candidatus Liberibacter asiaticus’ on Citrus Metabolism

Citation
Padhi et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ is associated with the devastating citrus disease Huanglongbing (HLB). It is transmitted by grafting infected material to healthy plants and by the feeding of the Asian citrus psyllid (Diaphorina citri). Previously, we demonstrated that a metabolomics approach using proton-nuclear magnetic resonance spectroscopy discriminates healthy from diseased plants via grafting. This work assessed the capability of this technology in discriminating healthy and diseased

Transcriptome Profiling of ‘Candidatus Liberibacter asiaticus’ in Citrus and Psyllids

Citation
De Francesco et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (Las) is an emergent bacterial pathogen that is associated with the devastating citrus huanglongbing (HLB). Vectored by the Asian citrus psyllid, Las colonizes the phloem tissue of citrus, causing severe damage to infected trees. So far, cultivating pure Las culture in axenic media has not been successful, and dual-transcriptome analyses aiming to profile gene expression in both Las and its hosts have a low coverage of the Las genome because of the low abunda