Phytopathology®


Publications
80

The Total Population Size of ‘Candidatus Liberibacter asiaticus’ Inside the Phloem of Citrus Trees and the Corresponding Metabolic Burden Related to Huanglongbing Disease Development

Citation
N.C. Vasconcelos et al. (2021). Phytopathology® 111 (7)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (CLas) is the predominant causal agent of citrus huanglongbing, the most devastating citrus disease worldwide. CLas colonizes phloem tissue and causes phloem dysfunction. The pathogen population size in local tissues and in the whole plant is critical for the development of disease symptoms by determining the load of pathogenicity factors and metabolic burden to the host. However, the total population size of CLas in a whole plant and the ratio of CLas to cit

Spatiotemporal Dynamics of ‘Candidatus Liberibacter asiaticus’ Colonization Inside Citrus Plant and Huanglongbing Disease Development

Citation
Pandey et al. (2021). Phytopathology® 111 (6)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (CLas), the causal agent of citrus huanglongbing (HLB), colonizes inside the phloem and is naturally transmitted by the Asian citrus psyllid (ACP). Here, we investigated spatiotemporal CLas colonization in different tissues after ACP transmission. Of the nine plants successfully infected via ACP transmission, CLas was detected in the roots of all trees at 75 days postremoval of ACPs (DPR) but in the mature leaf of only one tree; this finding is consistent wit

Assessment of the Effect of Thermotherapy on ‘Candidatus Liberibacter asiaticus’ Viability in Woody Tissue of Citrus via Graft-Based Assays and RNA Assays

Citation
Thapa et al. (2021). Phytopathology® 111 (5)
Names
Ca. Liberibacter asiaticus
Abstract
In 2019, citrus production in Florida declined by more than 70%, mostly because of Huanglongbing (HLB), which is caused by the bacterium ‘Candidatus Liberibacter asiaticus’ (CLas). Thermotherapy for HLB-affected trees was proposed as a short-term management solution to maintain field productivity. It was hypothesized that thermotherapy could eliminate HLB from affected branches; therefore, the study objectives were to show which time–temperature combinations eliminated CLas from woody tissues.

Two Unique Prophages of ‘Candidatus Liberibacter asiaticus’ Strains from Pakistan

Citation
Cui et al. (2021). Phytopathology® 111 (5)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (CLas) is a pathogen causing Huanglongbing (HLB, yellow shoot disease), which is highly destructive to citrus production. The CLas strains harbor prophages. We identified two unique prophages, designated as P-PA19-1 and P-PA19-2, in CLas strain PA19 from Pakistan using next-generation sequencing analysis. P-PA19-1 prophage has high sequence similarity (identity: 78.23%) at the early-gene region of prophage SC1 (Type 1), but it is significantly divergent in th

Detection and Molecular Characterization of ‘Candidatus Liberibacter asiaticus’ and Citrus Tristeza Virus Associated with Citrus Decline in Bhutan

Citation
Ghosh et al. (2021). Phytopathology® 111 (5)
Names
Ca. Liberibacter asiaticus
Abstract
Citrus, mainly mandarin (Citrus reticulata Blanco), is an economically important fruit crop in Bhutan. Despite having favorable agroclimatic conditions for citrus cultivation, the early decline of fruit-bearing orchards coupled with low crop productivity is a major concern among citrus growers. During a recent survey, an association of ‘Candidatus Liberibacter asiaticus’ (citrus greening) and citrus tristeza virus (CTV), either singly or as mixed infections in declined citrus trees, was recorde

Lso-HPE1, an Effector of ‘Candidatus Liberibacter solanacearum’, Can Repress Plant Immune Response

Citation
Levy et al. (2020). Phytopathology® 110 (3)
Names
“Liberibacter solanacearum”
Abstract
‘Candidatus Liberibacter solanacearum’ is a plant pathogen affecting the families Solanaceae and Apiaceae in different parts of the world. ‘Ca. L. solanacearum’ is a Gram-negative, fastidious α-proteobacterium that is vectored by different psyllid species. Plant-pathogenic bacteria are known for interfering with the host physiology or defense mechanisms, often by secreting bacterial effectors. Effector proteins are critical for virulence; therefore, the identification of effectors could help wi

Murraya paniculata and Swinglea glutinosa as Short-Term Transient Hosts of ‘Candidatus Liberibacter asiaticus’ and Implications for the Spread of Huanglongbing

Citation
Cifuentes-Arenas et al. (2019). Phytopathology® 109 (12)
Names
Ca. Liberibacter asiaticus
Abstract
Murraya paniculata and Swinglea glutinosa are aurantioid hosts of the Asian citrus psyllid (ACP) Diaphorina citri, the principal vector of ‘Candidatus Liberibacter asiaticus’ (Las). Las is the pathogen associated with huanglongbing (HLB), the Asian form of which is the most devastating disease of Citrus species and cultivars (Rutaceae: Aurantioideae). M. paniculata is a common ornamental and S. glutinosa is grown as an ornamental, a citrus rootstock, and a hedgerow fence plant. Because of the u

The in Planta Effective Concentration of Oxytetracycline Against ‘Candidatus Liberibacter asiaticus’ for Suppression of Citrus Huanglongbing

Citation
Li et al. (2019). Phytopathology® 109 (12)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB) or greening currently is the most devastating citrus disease worldwide. The fastidious phloem-colonizing bacterium ‘Candidatus Liberibacter asiaticus’ (CLas) is the causal agent of citrus HLB in Florida. Bactericides containing the active ingredient oxytetracycline (OTC) have been used in foliar spray to control citrus HLB in Florida since 2016. However, the minimum concentration of OTC required to suppress CLas in planta remains unknown. We developed a new method for evalua

Liberibacter crescens Is a Cultured Surrogate for Functional Genomics of Uncultured Pathogenic ‘Candidatus Liberibacter’ spp. and Is Naturally Competent for Transformation

Citation
Jain et al. (2019). Phytopathology® 109 (10)
Names
Liberibacter
Abstract
‘Candidatus Liberibacter’ spp. are uncultured insect endosymbionts and phloem-limited bacterial plant pathogens associated with diseases ranging from severe to nearly asymptomatic. ‘Ca. L. asiaticus’, causal agent of Huanglongbing or citrus “greening,” and ‘Ca. L. solanacearum’, causal agent of potato zebra chip disease, respectively threaten citrus and potato production worldwide. Research on both pathogens has been stymied by the inability to culture these agents and to reinoculate into any h