Methanocrinis natronophilus


Formal styling
Methanocrinis natronophilus Khomyakova et al., 2023 (valid 2024)
Effective publication
Khomyakova et al., 2023
SeqCode status
Valid (SeqCode)
Register List (validated)
Cannonical URL


N.L. pref. natrono-, pertaining to soda; N.L. masc. philus, friend, loving; N.L. masc. adj. natronophilus, soda-loving
Nomenclatural type
NCBI Assembly: GCA_029167045.1
Reference strain
Nomenclatural status
Validly published under the SeqCode


Cells are non-motile, rod-shaped, 1.9–4.8 x 0.6–1.0 μm. Forms multicellular filaments in a common sheath. Forms methane exclusively from acetate by the aceticlastic pathway. Obligately alkaliphilic with the pH range for growth from 7.5–7.8 to 10.2 (optimum at 9.3–9.5). NaCl is not required for growth, but up to 1 M total Na+ in the form of sodium carbonates is tolerated. The nongrowing cells still actively produce methane at pH up to 10.5 and 1.5 M total Na+. Ammonium serves as the nitrogen source. Optimal growth temperature is 35oC. Yeast extract is not essential for growth but slightly stimulatory. The complete genome of strain MxTs, available under the GenBank assembly accession number (GCA_029167045) is the designated nomenclatural type for the species and was recovered from an enrichment culture, cultivated on acetate and established from a saline soda lake, in southwestern Siberia, Russia. The genome of the type strain is 2.41 Mb with the G+C content of 58.18 mol%. Completeness is estimated by CheckM at 97.04% with 0.00% contamination. The GenBank accession number for the 16S rRNA gene sequence of MxTs is KP205578.
Archaea » Halobacteriota » Methanosarcinia » Methanotrichales » Methanotrichaceae » Methanocrinis » Methanocrinis natronophilus
Methanocrinis gtdb


NCBI Assembly:GCA_029167045.1
Enrichment Genome
Estimated Quality Metrics
  • Completeness: 97.04%
  • Contamination: 0.0%
  • Quality: 97.04
Ribosomal and transfer RNA genes
  • 1 16S rRNA (up to 100.0%)
  • 1 23S rRNA (up to 100.0%)
  • tRNAs for 17 amino acids
Sequencing depth
200.0 ×
Other features
  • G+C Content: 58.0%
  • Coding Density: 84.55%
  • Codon Table: 11
  • N50: 45,186 bp
  • Contigs: 100
  • Largest Contig: 107,349 bp
  • Assembly Length: 2,412,901 bp
  • Ambiguous Assembly Fraction: 0.0%
Submitter comments
Completeness and contamination were evaluated using CheckM (Parks et al., 2015).
Annotation was made using PGAP (Tatusova et al., 2016).
tRNA elements were detected by tRNAscan-SE (Lowe and Eddy, 1997).
Automated checks
See additional details


Outside links and data sources
Search sequences
Local history
Registered by
Merkel, Alexander 10 months ago
Submitted by
Merkel, Alexander 6 months ago
Validated by
Rodriguez-R, Luis M 5 months ago
Date of priority
2024-01-07 10:19 AM (UTC)


Citation Title
Khomyakova et al., 2023, Frontiers in Microbiology Phenotypic and genomic characterization of the first alkaliphilic aceticlastic methanogens and proposal of a novel genus Methanocrinis gen.nov. within the family Methanotrichaceae
Khomyakova et al., 2023, Frontiers in Microbiology Phenotypic and genomic characterization of Bathyarchaeum tardum gen. nov., sp. nov., a cultivated representative of the archaeal class Bathyarchaeia
Effective publication

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