Cells are long rods (0.2–0.3 μm in width, 6–10 μm in length), Gram-negative and motile by flagella. The temperature and pH ranges for growth are 16–45°C and pH 6.5–8, and optimal growth occurs at 37°C and pH 7.0. The strain requires sodium chloride for growth at a concentration range of 12.5–150 g/L (optimal at 25–75 g/L). Substrates used as carbon sources are arabinose, D-fructose, galactose, glucose, xylose, maltose, D-mannose, rhamnose, alginate, sucrose, xylan, starch, lignin, and carboxymethylcellulose (CMC). The products of glucose fermentation are acetate, ethanol, lactate, butyrate, hydrogen, and carbon dioxide. The predominant membrane fatty acids comprise 15:0 anteiso (23.71%), 14:0 (17.93%), 15:0 iso (12.02%), and 16:0 (9.99%). The composition of polar lipids was diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), unidentified phosphoglycolipids (PGL), and two unidentified glycolipids (GL). Strain NS-1 is sensitive to ampicillin, erythromycin, and rifampicin, while resistant to vancomycin, kanamycin, gentamicin, chloramphenicol, and streptomycin. The type strain NS-1(T) (KCTC 15988(T) = MCCC 1K04439(T)) was isolated from deep-sea sediment of cold seep, China, and deposited in Marine Culture Collection of China and Korea Collection for Type Cultures.