Type strain is Limnohabitans kasalickyi MaE-M4 (GCA_965234325.1), isolated from 5 m depth from Lake Maggiore, Italy (date: 2019-04-09), via high-throughput dilution to extinction cultivation. MaE-M4 has a genome size of 3.7 Mbp with a genomic GC content of 56.3%, contains 6 rRNA genes and 44 tRNAs. The genome is a high-quality draft consisting of 16 contigs. The genome contains genes encoding anoxygenic aerobic phototrophy (pufABLM). Genes for flagellar and pilus assembly were annotated. Pathways for cyanate, urea, and taurine degradation, thiosulfate oxidation (Sox pathway), methane/alkanesulfonate and glycolate oxidation, and the biosynthesis of all amino acids except for aspartate were predicted. Further, pathways for thiamine, riboflavin, pantothenate, coenzyme A, pimeloyl-ACP, THF, and heme biosynthesis were identified. The closest cultivated relative is Limnohabitans sp. Jir61 (GCF_003063545.1), with an average amino acid identity of 77.9% and average nucleotide identity of 80.1%. Current GTDB classification (R220): d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Burkholderiales; f__Burkholderiaceae_B; g__Limnohabitans; s__Limnohabitans sp002778325.