Type strain is Caulobacter lacus MiH-16 (GCA_965234345.1), isolated from 15 m depth from Lake Milada, Czechia (date: 2019-07-23), via high-throughput dilution to extinction cultivation. MiH-16 has a genome size of 3.6 Mbp with a genomic GC content of 67.6%, contains 3 rRNA genes and 47 tRNAs. The genome is a high-quality draft consisting of 5 contigs. The genome contains genes encoding rhodopsins. Genes for flagellar assembly and chemotaxis were annotated in the genome. Pathways for taurine degradation, assimilatory sulfate reduction and methane/alkanesulfonate oxidation and the biosynthesis of all amino acids were predicted. Further, pathways for thiamine, riboflavin, NAD, coenzyme A, pimeloyl-ACP, THF, and heme biosynthesis were identified. The closest cultivated relative is Caulobacter sp. Root1455 (GCF_001426905.1), with an average amino acid identity of 64% and average nucleotide identity of 74.7%. Current GTDB classification (R220): d__Bacteria; p__Pseudomonadota; c__Alphaproteobacteria; o__Caulobacterales; f__Caulobacteraceae; g__Caulobacter; s__Caulobacter sp903858185.