Type strain is Leadbetterella lacustris RE-19 (GCA_965234335.1), isolated from 0.5 m depth from the Římov reservoir, Czechia (date: 2019-08-05), via high-throughput dilution to extinction cultivation. RE-19 has a genome size of 5 Mbp with a genomic GC content of 37.48 %, contains 9 rRNA genes and 36 tRNAs. The genome is a high-quality draft consisting of 36 contigs. The genome contains genes encoding rhodopsins. Genes for gliding motility (gldBDHJKLMN, sprA) were annotated in the genome. Pathways for assimilatory sulfate reduction, methane/alkanesulfonate oxidation and the biosynthesis of all amino acids were predicted. Further, pathways for riboflavin, NAD, coenzyme A, THF, menaquinone, and heme biosynthesis were identified. The closest cultivated relative is Lacihabitans soyangensis KCTC23259 (GCF_024343775.1), with an average amino acid identity of 85.5% and average nucleotide identity of 82.97%. Lacihabitans is grouped with the genus Leadbetterella in GTDB. Current GTDB classification (R220): d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Cytophagales; f__Spirosomaceae; g__Leadbetterella; s__Leadbetterella fluviale_A.