Fontibacterium oligotrophicum


Citation

Formal styling
Fontibacterium oligotrophicum Fernandes et al., 2025
Effective publication
Fernandes et al., 2025
SeqCode status
Valid (SeqCode)
Register List
seqco.de/r:gnukuc44 (validated)
Canonical URL
https://seqco.de/i:49876

Nomenclature

Rank
Species
Syllabication
o.li.go.tro'phi.cum
Etymology
Gr. masc. adj. oligo, little; Gr. masc. adj. trophikos, nursing, tending; N.L. neut. adj. oligotrophicum, oligotrophic, referring to the low nutrient content of the isolation sites and the high abundance in oligotrophic lakes.
Nomenclatural type
NCBI Assembly: GCA_965235975.1
Nomenclatural status
Validly published under the SeqCode

Taxonomy

Description
Type genome is Fontibacterium oligotrophicum N-Balt2-05jul22-047 (GCA_965235975.1), a metagenome-assembled genome (MAG) assembled from 0.5 m depth from the slightly brackish part of Vistula Lagoon, Poland (date: 2022-07-05). N-Balt2-05jul22-047 has a genome size of 0.92 Mbp with a genomic GC content of 29.4% and contains 30 tRNAs. The genome is of high quality, consisting of 3 contigs, with a completeness of 96.4%, contamination of 0% and strain heterogeneity of 0% as assessed with checkM. The metagenome was assembled with FLYE from combined long- and short-read sequencing (Oxford Nanopore and Illumina NovaSeq). Metagenomic fragment recruitment of >600 samples from five continents indicate that the species is highly abundant in oligotrophic lakes in temperate and subtropical regions. The closest cultivated relatives are Fontibacterium commune, syn. ‘Candidatus Fonsibacter ubiquis’ LSUCC0530 (GCF_002688585.1; later reclassified to ‘Ca. Allofontibacter communis’), with an average amino acid identity of 92.57% and average nucleotide identity of 90.07% and another newly proposed species, Fontibacterium medardicus ME-17 (GCA_965235075.1), with an AAI of 85.45% and an ANI of 88.16%. Current GTDB classification (R220): d__Bacteria; p__Pseudomonadota; c__Alphaproteobacteria; o__Pelagibacterales; f__Pelagibacteraceae; g__Fonsibacter; s__Fonsibacter sp947497305.
Classification
Bacteria » Pseudomonadota » Alphaproteobacteria » Pelagibacterales » Pelagibacteraceae » Fontibacterium » Fontibacterium oligotrophicum
Parent
Fontibacterium

Genomics

Accession
NCBI Assembly:GCA_965235975.1
Type
Metagenome-Assembled Genome (MAG)
Estimated Quality Metrics
  • Completeness: 96.4%
  • Contamination: 0.0%
  • Quality: 96.4
Ribosomal and transfer RNA genes
  • 0 16S rRNAs
  • 0 23S rRNAs
  • tRNAs for 18 amino acids
Sequencing depth
108.067 ×
Source
Other features
  • G+C Content: 29.4%
  • Coding Density: 93.12%
  • Codon Table: 11
  • N50: 461,927 bp
  • Contigs: 3
  • Largest Contig: 461,927 bp
  • Assembly Length: 923,333 bp
  • Ambiguous Assembly Fraction: 0.0%
Submitter comments
This is a metagenome-assembled genome (MAG) that was assembled with FLYE v2.9.1-b1780 (settings: --nano-corr –meta –no-alt-contigs) from combined long- and short-read sequencing (Oxford Nanopore and Illumina NovaSeq). Contigs ≥3 kbp were used for hybrid binning (tetranucleotide frequencies and coverage data) using MaxBin-2.2.7, MetaBAT and MetaBAT2 and DAS Tool-1.1.3 to combine their predictions. Bins were manually curated and CheckM v1.1.3 was used to estimate bin completeness, contamination, and strain heterogeneity. rRNA genes were predicted with barrnap v0.9, tRNA genes were predicted with tRNAscan v1.4. Although the fragmentation of this long-read MAG is low (it consists of only 3 contigs) and it is highly complete (96.4%), it does not contain any rRNA genes. 
Automated checks
Complete

Last modified 4 months ago

Metadata

Outside links and data sources
Search sequences
Local history
Registered
8 months ago by Salcher, Michaela M
Submitted
3 months ago by Salcher, Michaela M
Curators
Validated
2 months ago by Rodriguez-R, Luis M
Date of priority
2025-08-28 03:13 PM (UTC)

Publications
1

Citation Title
Fernandes et al., 2025, Nature Microbiology Ecophysiology and global dispersal of the freshwater SAR11-IIIb genus Fontibacterium
Effective publication



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