Fontibacterium temperatum


Citation

Formal styling
Fontibacterium temperatum Fernandes et al., 2025
Effective publication
Fernandes et al., 2025
SeqCode status
Valid (SeqCode)
Register List
seqco.de/r:gnukuc44 (validated)
Canonical URL
https://seqco.de/i:49881

Nomenclature

Rank
Species
Syllabication
tem.pe.ra'tum
Etymology
L. neut. adj. temperatum, referring to temperate climate, the species mainly occurs in lakes of the temperate region of the Northern Hemisphere
Nomenclatural type
NCBI Assembly: GCA_964203055.1
Nomenclatural status
Validly published under the SeqCode

Taxonomy

Description
Type genome is Fontibacterium temperatum ZE-03apr19-LR-3 (GCA_964203055.1), a metagenome-assembled genome (MAG) assembled from 5 m depth from Lake Zurich, Switzerland (date: 2019-04-03). ZE-03apr19-LR-3 has a genome size of 0.9 Mbp with a genomic GC content of 29.4% and contains 28 tRNAs. The genome is of high quality, consisting of 3 contigs, with a completeness of 94%, contamination of 0% and strain heterogeneity of 0% as assessed with checkM. The metagenome was assembled with FLYE from combined long-and short-read sequencing (Oxford Nanopore and Illumina NovaSeq). Metagenomic fragment recruitment of >600 samples from five continents indicate that the species is highly abundant in temperate lakes of the Northern Hemisphere. The closest cultivated relatives are Fontibacterium commune, syn. ‘Candidatus Fonsibacter ubiquis’ LSUCC0530 (GCF_002688585.1; later reclassified to ‘Ca. Allofontibacter communis’), with an average amino acid identity of 87.44% and average nucleotide identity of 85.23% and another newly proposed species, Fontibacterium abundans MiE-29 (GCA_965235095.1), with an AAI of 93.82% and an ANI of 92.5%. Current GTDB classification (R220): d__Bacteria; p__Pseudomonadota; c__Alphaproteobacteria; o__Pelagibacterales; f__Pelagibacteraceae; g__Fonsibacter; s__Fonsibacter sp000510845.
Classification
Bacteria » Pseudomonadota » Alphaproteobacteria » Pelagibacterales » Pelagibacteraceae » Fontibacterium » Fontibacterium temperatum
Parent
Fontibacterium

Genomics

Accession
NCBI Assembly:GCA_964203055.1
Type
Metagenome-Assembled Genome (MAG)
Estimated Quality Metrics
  • Completeness: 94.0%
  • Contamination: 0.0%
  • Quality: 94.0
Ribosomal and transfer RNA genes
  • 0 16S rRNAs
  • 0 23S rRNAs
  • tRNAs for 18 amino acids
Sequencing depth
10.0 ×
Source
Other features
  • G+C Content: 29.4%
  • Coding Density: 95.14%
  • Codon Table: 11
  • N50: 585,694 bp
  • Contigs: 3
  • Largest Contig: 585,694 bp
  • Assembly Length: 937,461 bp
  • Ambiguous Assembly Fraction: 0.0%
Submitter comments
This is a metagenome-assembled genome (MAG) that was assembled with FLYE v2.9.1-b1780 (settings: --nano-corr –meta –no-alt-contigs) from combined long- and short-read sequencing (Oxford Nanopore and Illumina NovaSeq). Contigs ≥3 kbp were used for hybrid binning (tetranucleotide frequencies and coverage data) using MaxBin-2.2.7, MetaBAT and MetaBAT2 and DAS Tool-1.1.3 to combine their predictions. Bins were manually curated and CheckM v1.1.3 was used to estimate bin completeness, contamination, and strain heterogeneity. Sequencing depth was not estimated and thus set to 10x. rRNA genes were predicted with barrnap v0.9, tRNA genes were predicted with tRNAscan v1.4. Although the fragmentation of this long-read MAG is low (it consists of only 3 contigs), and it is highly complete (94%), it does not contain any rRNA genes. 
Automated checks
Complete

Last modified 7 months ago

Metadata

Outside links and data sources
Search sequences
Local history
Registered
8 months ago by Salcher, Michaela M
Submitted
3 months ago by Salcher, Michaela M
Curators
Validated
2 months ago by Rodriguez-R, Luis M
Date of priority
2025-08-28 03:13 PM (UTC)

Publications
1

Citation Title
Fernandes et al., 2025, Nature Microbiology Ecophysiology and global dispersal of the freshwater SAR11-IIIb genus Fontibacterium
Effective publication



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