The present study was carried out to clarify the taxonomic assignment of two closely related
Amycolatopsis
species. Genomic information for 48 type strains was available at the time of conducting this analysis. Our analysis showed that two species, viz.
Amycolatopsis eurytherma
Kim et al. 2002 and
Amycolatopsis thermoflava
Chun et al. 1999, are conspecific. The 16S rRNA gene sequences of the two species possess 98.85 % sequence similarity. Further, whole-genome comparisons showed that
A. eurytherma
DSM 44348T and
A. thermoflava
N1165T shared 98.75 % average nucleotide identity, 98.63 % average amino acid identity and 87.8 % digital DNA–DNA hybridization values. These values exceed the threshold values for bacterial species delineation, indicating that they belong to the same species. Further, the phylogenomic analysis based on the core genome of the strains under study confirmed that
A. eurytherma
DSM 44348T and
A. thermoflava
N1165T formed a monophyletic clade. Based on this evidence we propose the reclassification of
Amycolatopsis eurytherma
Kim et al. 2002 as a later heterotypic synonym of
Amycolatopsis thermoflava
Chun et al. 1999.