Publications

4604

Abstract Bacterial symbionts were shown to have significant beneficial impacts on the fitness of their host in insects, but little is known on the symbionts of spiders and their interactions with their hosts. Here, we assembled and investigated the circular 575 kb genome of Candidatus Argioplasma dusa, a novel bacterial symbiont of the wasp spider Argiope bruennichi . Phylogenomic analysis placed this species within the phylum Tenericutes, in a poorly characterized clade that may represent a new order-level lineage or affiliate with the Mycoplasmatales order. With 559 predicted genes, the genome is relatively small compared to other Tenericutes genomes (on average 969 genes) and has a low GC content of ~24%. While the genome encodes genes for proteins involved in glycolysis and fermentative acetate production, it revealed minimal biosynthetic capabilities with pathways for nucleotide, amino acid and vitamin biosynthesis being absent in Ca . Argioplasma dusa. This suggests an intracellular endosymbiotic lifestyle within the spider host. The symbiont was detected in A. bruennichi populations across the distribution range of the spider but appears to be absent in certain populations.

‘Candidatus Liberibacter asiaticus’ (CLas), the causal agent of citrus huanglongbing, is transmitted by the Asian citrus psyllid Diaphorina citri. While CLas-positive (CLas+) females exhibit increased fecundity and metabolic demands, their neuroendocrine regulation mechanisms remain unclear. We propose CLas manipulates dopamine (DA) signaling to enhance psyllid fecundity and CLas proliferation. Metabolomics revealed elevated DA in CLas+ females. Silencing DA synthesis genes and receptor DcDop2 via RNAi reduced lipid reserves, fecundity, and ovarian CLas titers. Through combined in vivo and in vitro experiments, we demonstrated that the microRNA miR-31a suppresses DcDop2 expression by binding to its 3’ untranslated region. Overexpression of miR-31a resulted in decreased DcDop2 expression and CLas titers in the ovaries, eliciting phenotypic defects akin to DcDop2 knockdown. Furthermore, DcDop2 knockdown and miR-31a overexpression reduced juvenile hormone (JH) levels and adipokinetic hormone (AKH) signaling in fat bodies and ovaries. Consequently, CLas regulates the DA-DcDop2 signaling axis to improve D. citri lipid metabolism and fecundity, while simultaneously promoting its replication. These findings reveal a coevolution between CLas proliferation and ovarian development in the insect host. This discovery enhances our understanding of the molecular interplay between plant pathogens and vector insects and offers novel targets and strategies for HLB field management.

In 2024, an infestation of corn leafhoppers, Dalbulus maidis (DeLong) (Hemiptera: Cicadellidae), was detected in several commercial corn fields across Oklahoma (OK) (Faris et al. 2024). Corn hybrids in the infested field showed symptoms resembling maize bushy stunt, including chlorotic striping, reddening of leaves, production of multiple ears, and stunting. Visual disease incidence ranged from 25% to 100% among affected fields. Leaf samples from symptomatic plants were collected from 15 commercial corn fields across seven counties in OK (Payne, Logan, Caddo, Kay, Texas, Pottawatomie, and Grady) during September, October, and November of 2024. Each sample consisted of 3-4 symptomatic corn leaves randomly collected in each field. In addition, 43 corn leafhopper samples were collected from the symptomatic fields using handheld vacuums. Each corn leafhopper sample consisted of a minimum of 10 corn leafhoppers per field. In the laboratory, the corn leafhoppers were identified as D. maidis based on morphological characteristics as described (Faris et al. 2024). DNA from the 15 leaf samples was extracted from the leaf midrib and blade tissues using the DNeasy® Plant Mini Kit. Insect DNA was extracted using a CTAB-based method (Gorayeb et al. 2023), with each sample consisting of five pooled insects per field. DNA samples from plants and insects were initially screened using the R16F2n/R16R2 primer pair (Gundersen and Lee, 1996) for the presence of phytoplasmas. Seventeen insect and five plant samples tested positive for phytoplasma. Subsequent phytoplasma screening was performed on insect and plant samples collected from the same fields on the same dates, comprising a total of four insect and five plant samples. To further characterize the phytoplasma, a semi-nested PCR targeting the 16S rRNA gene was performed on these samples (four insect and five plant samples) using primer pairs P1/16S-SR followed by P1A/16S-SR (Wei et al. 2021), yielding an expected amplicon of approximately 1.5 Kb. An additional gene marker, the ribosomal protein (rp) gene, was also amplified using universal primers rpF1/rpR1 (Lee et al. 1998), producing 1.2 Kb PCR amplicons. PCR products were subjected to Sanger sequencing, and 16S rRNA and rp gene sequences were deposited in GenBank under accession numbers PX927497–PX927505 and PX993703-PX993711, respectively. BLASTn analysis of the 16S rRNA sequences showed 99.14–99.93% nucleotide identity with reference strains of ‘Candidatus Phytoplasma asteris’ associated with maize bushy stunt (accessions MW578284 and CP015149). Similarly, the rp gene sequences shared 99.91–100% nucleotide identity with the same reference strains. Using the iPhyClassifier tool (Zhao et al. 2013), the 16S rRNA gene sequences were classified within 16Sr group I, subgroup B, with a similarity coefficient of 1.0 (AP006628). The pairwise comparison and subsequent phylogenetic analysis showed that the OK isolates clustered within the 16SrI-B clade. This finding represents the first report of a 16SrI-B phytoplasma (‘Candidatus Phytoplasma asteris’) associated with maize bushy stunt in OK. Although currently classified as ‘Candidatus Phytoplasma asteris’, a proposal to establish a novel phytoplasma species, ‘Candidatus Phytoplasma zeae’, has been made based on ecological distinctiveness, vector specificity, whole-genome comparisons, and community consensus (Pellegrinetti et al. 2025).

Abstract The pear psyllid Cacopsylla pyri is the main vector of ‘ Candidatus Phytoplasma pyri’, the causal agent of pear decline, a disease associated with severe yield losses in pear production. Phytoplasmas are further known to interfere with their vectors metabolism; therefore, we investigated whether an infection with ‘ Ca. P. pyri’ is associated with changes in nutrient storage of the pear psyllids. Additionally, we measured whether the phytoplasma infection is associated with differences in flight performance in C. pyri , as the flight activity of the vector is a key component of phytopathogen spread. Using a flight mill, we quantified flight distance, duration, and speed, and combined qPCR-based infection detection with colorimetric assays to determine protein, carbohydrate, and lipid content from the same individual. Infected psyllids contained significantly higher levels of soluble carbohydrates than uninfected individuals. Lipid content was not affected by the phytoplasma infections but was found to be consistently higher in males than in females, while protein levels did not vary with sex or infection status. Despite infection-related differences in carbohydrate levels, phytoplasma infection did not significantly affect flight duration, distance, or speed. These results indicate that infection-associated shifts in bulk carbohydrate storage do not translate into altered flight motivation or performance, suggesting a functional decoupling between metabolic state and realised dispersal capacity under standardised laboratory conditions.